Recombinant
RabMAb

Recombinant Anti-CD42b antibody [EPR19204] (ab210407)

Overview

  • Product name

    Anti-CD42b antibody [EPR19204]
    See all CD42b primary antibodies
  • Description

    Rabbit monoclonal [EPR19204] to CD42b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human CD42b aa 250-350. The exact sequence is proprietary.
    Database link: P07359

  • Positive control

    • WB: Human fetal spleen lysate. IHC-P: Human spleen, acute myelocytic leukemia and lung cancer tissues. IP: Human spleen whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19204
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 72 kDa).
IP 1/40.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • Antigen CD42b alpha antibody
    • BDPLT1 antibody
    • BDPLT3 antibody
    • BSS antibody
    • CD 42b antibody
    • CD42b alpha antibody
    • CD42B antibody
    • CD42b antigen antibody
    • DBPLT3 antibody
    • GLYCOCALICIN antibody
    • Glycoprotein Ib (platelet) alpha polypeptide antibody
    • Glycoprotein Ibalpha antibody
    • GP Ib alpha antibody
    • GP1B antibody
    • GP1BA antibody
    • GPIb alpha antibody
    • GPIbA antibody
    • MGC34595 antibody
    • Platelet glycoprotein Ib alpha chain antibody
    • Platelet glycoprotein Ib alpha polypeptide antibody
    • Platelet membrane glycoprotein 1b alpha subunit antibody
    • VWDP antibody
    see all

Images

  • All lanes : Anti-CD42b antibody [EPR19204] (ab210407) at 1/10000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 72 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on Human spleen platelets is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human acute myelocytic leukemia tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on megakaryocytes is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on lung cancer platelets is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • CD42b was immunoprecipitated from 1mg of Human spleen whole cell lysate with ab210407 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab210407 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human spleen whole cell lysate, 10µg (Input).

    Lane 2: ab210407 IP in Human spleen whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab210407 in Human spleen whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab210407 has not yet been referenced specifically in any publications.

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