Overview

  • Product name

    Anti-CD42b antibody [EPR6995]
    See all CD42b primary antibodies
  • Description

    Rabbit monoclonal [EPR6995] to CD42b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD42b aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human platelet lysate, Human spleen tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.21% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6995
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 143 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance

      CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
    • Cellular localization

      Cell Membrane
    • Database links

    • Alternative names

      • Antigen CD42b alpha antibody
      • BDPLT1 antibody
      • BDPLT3 antibody
      • BSS antibody
      • CD 42b antibody
      • CD42b alpha antibody
      • CD42B antibody
      • CD42b antigen antibody
      • DBPLT3 antibody
      • GLYCOCALICIN antibody
      • Glycoprotein Ib (platelet) alpha polypeptide antibody
      • Glycoprotein Ibalpha antibody
      • GP Ib alpha antibody
      • GP1B antibody
      • GP1BA antibody
      • GPIb alpha antibody
      • GPIbA antibody
      • MGC34595 antibody
      • Platelet glycoprotein Ib alpha chain antibody
      • Platelet glycoprotein Ib alpha polypeptide antibody
      • Platelet membrane glycoprotein 1b alpha subunit antibody
      • VWDP antibody
      see all

    Images

    • ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    • Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelets at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 69 kDa



      Diluting and blocking buffer: 5% NFDM/TBST

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with ab134087 at a dilution of 1/100.
    • Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelet lysate at 10 µg

      Secondary
      HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 69 kDa
      Observed band size: 143 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhao G  et al. Clinical diagnosis of adult patients with acute megakaryocytic leukemia. Oncol Lett 16:6988-6997 (2018). Read more (PubMed: 30546432) »
    • Saito H  et al. Potential of extravasated platelet aggregation as a surrogate marker for overall survival in patients with advanced gastric cancer treated with preoperative docetaxel, cisplatin and S-1: a retrospective observational study. BMC Cancer 17:294 (2017). IHC-P ; Human . Read more (PubMed: 28449652) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134087.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up