Recombinant
RabMAb

Recombinant Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)

Overview

  • Product name

    Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free
    See all CD42b primary antibodies
  • Description

    Rabbit monoclonal [EPR6995] to CD42b - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD42b aa 1-100.

  • Positive control

    • Human platelet lysate, Human spleen tissue
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6995
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 143 kDa (predicted molecular weight: 69 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance

      CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
    • Cellular localization

      Cell Membrane
    • Database links

    • Alternative names

      • Antigen CD42b alpha antibody
      • BDPLT1 antibody
      • BDPLT3 antibody
      • BSS antibody
      • CD 42b antibody
      • CD42b alpha antibody
      • CD42B antibody
      • CD42b antigen antibody
      • DBPLT3 antibody
      • GLYCOCALICIN antibody
      • Glycoprotein Ib (platelet) alpha polypeptide antibody
      • Glycoprotein Ibalpha antibody
      • GP Ib alpha antibody
      • GP1B antibody
      • GP1BA antibody
      • GPIb alpha antibody
      • GPIbA antibody
      • MGC34595 antibody
      • Platelet glycoprotein Ib alpha chain antibody
      • Platelet glycoprotein Ib alpha polypeptide antibody
      • Platelet membrane glycoprotein 1b alpha subunit antibody
      • VWDP antibody
      see all

    Images

    • ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134087).

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with ab134087 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134087).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134087).

    References

    This product has been referenced in:

    • Feng Y  et al. Platelets Direct Monocyte Differentiation Into Epithelioid-Like Multinucleated Giant Foam Cells With Suppressive Capacity Upon Mycobacterial Stimulation. J Infect Dis N/A:N/A (2014). IHC-P ; Human . Read more (PubMed: 24987031) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab221213.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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