Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Thrombus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Target Retrieval high pH, Dako
Permeabilization
No
Specification
Thrombus
Blocking step
(agent) for 30 minute(s) · Concentration: 100%
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Swine anti-rabbit IgG Dako P0399
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This marker works well for the detection of thrombus in FFPE tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Katja Eildermann
Verified customer
Submitted Aug 28 2018