Overview

  • Product name

    Anti-CD42d/GP5 antibody [1C2]
    See all CD42d/GP5 primary antibodies
  • Description

    Armenian hamster monoclonal [1C2] to CD42d/GP5
  • Host species

    Armenian hamster
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD42d/GP5.

  • Positive control

    • Mouse platelets
  • General notes

     This product was previously labelled as CD42d

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1C2
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105-8 cells.

Staining the cell sample in a final volume of 100 µl is recommended.

 

 

 

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
  • Tissue specificity

    Platelets and megakaryocytes.
  • Sequence similarities

    Contains 13 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 42d antibody
    • CD42d antibody
    • CD42d antigen antibody
    • Glycoprotein 5 antibody
    • Glycoprotein V antibody
    • Glycoprotein V platelet antibody
    • GlycoproteinV antibody
    • Gp 5 antibody
    • GP V antibody
    • GP5 antibody
    • GPV antibody
    • GPV_HUMAN antibody
    • Platelet glycoprotein V antibody
    • Platelet glycoprotein V precursor antibody
    see all

Images

  • Flow cytometric staining of mouse platelets with Anti-Mouse CD41 FITC and 0.125µg of Armenian Hamster IgG Isotype Control Purified (left) or 0.125µg of ab95780 (right) followed by Anti-Armenian Hamster IgG PE. Total events were used for analysis.

References

This product has been referenced in:

  • Sato N  et al. Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d). Hybridoma 19:455-61 (2000). Read more (PubMed: 11152397) »
  • Takada K  et al. Novel monoclonal antibody reactive with thrombin-sensitive 74-kDa glycoproteins present on platelets and megakaryocytes both from mouse and rat. Hybridoma 14:361-7 (1995). Read more (PubMed: 8522348) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your reply.

I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

I look forward to your reply and helping resolve this matter.

Read More

Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

Read More

Question
Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

Read More

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