Anti-CD42d/GP5 antibody [1C2] (ab95780)

Submit a review Q&A (4)References (2)
Flow Cytometry - Anti-CD42d/GP5 antibody [1C2] (ab95780)

    Key features and details

    • Armenian hamster monoclonal [1C2] to CD42d/GP5
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    Overview

    • Product name

      Anti-CD42d/GP5 antibody [1C2]
      See all CD42d/GP5 primary antibodies

    • Description

      Armenian hamster monoclonal [1C2] to CD42d/GP5

    • Host species

      Armenian hamster

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse, Rat

    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD42d/GP5.

    • Positive control

      • Mouse platelets

    • General notes

       This product was previously labelled as CD42d

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: 2.68% PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Clonality

      Monoclonal

    • Clone number

      1C2

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab95780 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.125-0.25µg for 105-8 cells.

    Staining the cell sample in a final volume of 100 µl is recommended.

     

     

     

    ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.

    • Tissue specificity

      Platelets and megakaryocytes.

    • Sequence similarities

      Contains 13 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.

    • Post-translational
      modifications

      The N-terminus is blocked.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 42d antibody
        • CD42d antibody
        • CD42d antigen antibody
        • Glycoprotein 5 antibody
        • Glycoprotein V antibody
        • Glycoprotein V platelet antibody
        • GlycoproteinV antibody
        • Gp 5 antibody
        • GP V antibody
        • GP5 antibody
        • GPV antibody
        • GPV_HUMAN antibody
        • Platelet glycoprotein V antibody
        • Platelet glycoprotein V precursor antibody
        see all

      Images

      • Flow Cytometry - Anti-CD42d/GP5 antibody [1C2] (ab95780)
        Flow Cytometry - Anti-CD42d/GP5 antibody [1C2] (ab95780)
        Flow cytometric staining of mouse platelets with Anti-Mouse CD41 FITC and 0.125µg of Armenian Hamster IgG Isotype Control Purified (left) or 0.125µg of ab95780 (right) followed by Anti-Armenian Hamster IgG PE. Total events were used for analysis.

      References (2)

      Publishing research using ab95780? Please let us know so that we can cite the reference in this datasheet.

      ab95780 has been referenced in 2 publications.

      • Sato N  et al. Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d). Hybridoma 19:455-61 (2000). PubMed: 11152397
      • Takada K  et al. Novel monoclonal antibody reactive with thrombin-sensitive 74-kDa glycoproteins present on platelets and megakaryocytes both from mouse and rat. Hybridoma 14:361-7 (1995). PubMed: 8522348

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      1-4 of 4 Q&A

      Question

      We have purchased some antibodies against rat platelets:

      Primary:

      ab108949 - mouse monoclonal anti CD63, FITC labeled
      (ab106163 as isotype control)

      ab54427 - mouse monoclonal anti CD62P
      (ab91353 as the isotype control, as a secondary antibody)

      ab95780 anti CD42d armenian hamster antibody
      (ab18479 as an isotype control, for which we are using APC-conjugated Goat Anti-Hamster IgG from Leinco).

      We are ibodies.doing flow cytometry experiments on fixed whole blood, and fixed platelet-rich plasma. The problem is that the samples with the isotype control show the same fluorescence as the samples with the primary ant We have tried a number of different fixing and staining protocols, with and without washing, but until now had no success. What I am wondering about is, how reliable are these primary antibodies for the use with rat platelets?

      Some help would be greatly appreciated.

      Read More
      Answer

      Thank you for your reply.

      I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

      To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

      I look forward to your reply and helping resolve this matter.

      Read More

      Question

      Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

      Feel free to contact him to double-check, he is the one who gave me the customer ID.

      Read More
      Answer

      Thank you for your reply.

      I have sent the quote for the products requested to the email address we have on file for **********.

      When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

      If there is anything else I can help you with, please let me know.

      Read More

      Question

      Concerning the quote, the customer number is**********

      Read More
      Answer

      Thank you for your reply.

      Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

      Thank you for your patience, and I look forward to your reply.

      Read More

      Question

      I selected the following antibodies from the ABcam catalog:

      Primary:

      Anti-CD63 antibody [AD1] (FITC)

      ab108949

      $337.00



      Anti-CD62P antibody [Psel.KO.2.7]

      ab54427

      $337.00



      Anti-CD42d antibody [1C2]

      ab95780

      $337.00



      Secondary:


      Goat anti-Mouse IgG H&L (Cy5.5 ®)

      ab6947

      $185.00



      Goat anti-Mouse IgG1 heavy chain (FITC)

      ab97239

      $165.00



      Goat polyclonal to Armenian Hamster (TR)

      ab5743

      $165.00



      Isotype controls:


      MOUSE IgG1 antibody [MOPC-21] (FITC)

      ab106163

      $325.00



      Mouse IgG1 [ICIGG1] - Isotype Control

      ab91353

      $315.00



      Armenian Hamster IgG monoclonal [HTK888] - Isotype control

      ab18479

      $325.00



      Two questions:

      For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

      If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

      Read More
      Answer

      Thank you for your reply.

      The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

      I look forward to your reply.

      Read More

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