Recombinant
RabMAb

Recombinant Anti-CD43 antibody [EPR21903] (ab235452)

Overview

  • Product name

    Anti-CD43 antibody [EPR21903]
    See all CD43 primary antibodies
  • Description

    Rabbit monoclonal [EPR21903] to CD43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD43 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P16150

  • Positive control

    • WB: HL-60, Ramos, IM-9 anf THP-1 whole cell lysates; Human colon and lung lysates. IHC-P: Human tonsil and B cell lymphoma tissue. ICC/IF: IM-9 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 40 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function

    One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificity

    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications

    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue labeling CD43 with ab235452 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human B cell lymphoma (PMID: 2794085) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD43 with ab235452 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the T cell zone and scattered T cells in the germinal center (PMID: 2794085) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IM-9 (human multiple myeloma B lymphoblast cell line) cells labeling CD43 with ab235452 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the IM-9 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red)

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-CD43 antibody [EPR21903] (ab235452) at 1/1000 dilution

    Lane 1 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : IM-9 (human multiple myeloma B lymphoblast cell line) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia cell line0 whole cell lysate
    Lane 5 : Human colon lysate
    Lane 6 : Human lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa
    Observed band size: 100-150,20,50-60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1-4: 15 seconds; Lanes 5 & 6: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMIDs: 2794085, 23987472 and 7683406). We also observe additional bands at or below 20 kDa in some cell lines, which could be due to  proteolysitic cleavage of the protein (PMIDs: 18586676 and 15540986).

References

ab235452 has not yet been referenced specifically in any publications.

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