Alexa Fluor® 700 Anti-CD43 antibody [MEM-59] (ab270666)
Key features and details
- Alexa Fluor® 700 Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 700
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 700 Anti-CD43 antibody [MEM-59]
See all CD43 primary antibodies -
Description
Alexa Fluor® 700 Mouse monoclonal [MEM-59] to CD43 -
Host species
Mouse -
Conjugation
Alexa Fluor® 700 -
Specificity
This antibody recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD43. Human T lymphocytes.
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Positive control
- Flow Cyt: Human peripheral blood.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 8.0
Preservative: 0.0975% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-59 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-Transferrin Receptor antibody [MEM-75] (ab18242)
- APC/Cy7® Anti-Transferrin Receptor antibody [MEM-75] (ab233254)
- FITC Anti-Transferrin Receptor antibody [MEM-75] (ab239251)
- PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75] (ab239340)
- Biotin Anti-Transferrin Receptor antibody [MEM-75] (ab28116)
- APC Anti-Transferrin Receptor antibody [MEM-75], prediluted (ab64672)
- Anti-Transferrin Receptor antibody [MEM-75] (ab9179)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension.
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Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270666 has not yet been referenced specifically in any publications.