Immunocytochemistry/ Immunofluorescence abreview for Anti-CD43 antibody [MEM-59] (FITC)

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cells)
Specification
embryonic stem cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Other product details

Dilution
1/5
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 10% FCS

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
We expected CD43 to be expressed in ES cells but failed to detect it. Unclear if this is due to the antibody or absence of the protein.

The image has been pseudocolored to red instead of green.
Abcam response
Thank you very much for sharing this data with us. We are currently unsure about the level of CD43 protein expression seen in ES cells. So, either the protein expression level is too low/absent or this antibody does not work in ICC/IF, under these conditions. Abcam would welcome feedback from other customers using this antibody in ICC/IF.

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Submitted May 11 2011

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