We expected CD43 to be expressed in ES cells but failed to detect it. Unclear if this is due to the antibody or absence of the protein.
The image has been pseudocolored to red instead of green.
Thank you very much for sharing this data with us. We are currently unsure about the level of CD43 protein expression seen in ES cells. So, either the protein expression level is too low/absent or this antibody does not work in ICC/IF, under these conditions. Abcam would welcome feedback from other customers using this antibody in ICC/IF.
Abcam user community
Submitted May 11 2011
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