Anti-CD43 antibody [SPN/839] - BSA and Azide free (ab212850)
Key features and details
- Mouse monoclonal [SPN/839] to CD43 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD43 antibody [SPN/839] - BSA and Azide free
See all CD43 primary antibodies -
Description
Mouse monoclonal [SPN/839] to CD43 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD43 aa 1 to the C-terminus.
Database link: P16150 -
Positive control
- Paracortex in a tonsil or a reactive lymph node; human spleen tissue; human spleen lysate. ICC: K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SPN/839 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(for 30 minutes at RT) |
|
ICC |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (for 30 minutes at RT) |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
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Immunofluorescence Analysis of K562 cells labeling CD43 with ab212850 followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot Live 650.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue labeling CD43 with ab212850 at 1µg/mL.
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Anti-CD43 antibody [SPN/839] - BSA and Azide free (ab212850) at 1 µg/ml + Human spleen tissue lysate
Predicted band size: 40 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab212850 has not yet been referenced specifically in any publications.