Overview

  • Product name

    Anti-CD43 antibody [W3/13]
    See all CD43 primary antibodies
  • Description

    Mouse monoclonal [W3/13] to CD43
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD43. Rat thymocyte membrane.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat spleen tissue. IHC-P: Rat spleen tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab22351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 0.2 µg/ml.

Target

  • Function

    One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificity

    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications

    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

Images

  • IHC image of CD43 staining in a section of formalin-fixed paraffin-embedded normal rat spleen* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22351, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from Charles River.

  • IHC image of CD43 staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab22351 (shown in red) at 1µg/ml and co-localization of T cells stained using ab16669 (shown in green), Rabbit monoclonal to CD3, at 1/150 dilution. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®647, 1/1000)) and ab150077 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®488, 1/1000)) for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

  • Lewis rat splenocytes stained with ab22351 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22351) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

References

This product has been referenced in:

  • De Miguel C  et al. High dietary protein exacerbates hypertension and renal damage in Dahl SS rats by increasing infiltrating immune cells in the kidney. Hypertension 57:269-74 (2011). IHC-P ; Rat . Read more (PubMed: 21173345) »
  • Skoumal R  et al. Parthenolide inhibits STAT3 signaling and attenuates angiotensin II-induced left ventricular hypertrophy via modulation of fibroblast activity. J Mol Cell Cardiol 50:634-41 (2011). IHC-P ; Rat . Read more (PubMed: 21223972) »
See all 4 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. I have contacted the originator of the product but unfortunately, no epitope mapping studies have been performed with this antibody. It seems that it has never been shown that the antibody recognizes a linear epitope. Having said that however, there is a reference which indicates W3/13 as recognising a different epitope to HIS17 which may indicate some studies have been done. The reference is: Ahuja V, Miller SE, Howell DN. Identification of two subpopulations of rat monocytes expressing disparate molecular forms and quantities of CD43. Cell Immunol. 1995 Jun;163(1):59-69. Unfortunately, I do not have access to this publication but I hope it will provide some useful information. If there is anything else that I can help you with, please do not hesitate to contact me.

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