Key features and details
- Mouse monoclonal [W3/13] to CD43
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-CD43 antibody [W3/13]
See all CD43 primary antibodies
DescriptionMouse monoclonal [W3/13] to CD43
Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cytmore details
Species reactivityReacts with: Rat
Tissue, cells or virus corresponding to Rat CD43. Rat thymocyte membrane.
- Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat spleen tissue. IHC-P: Rat spleen tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab22351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
- Information by UniProt
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
IHC image of CD43 staining in a section of formalin-fixed paraffin-embedded normal rat spleen* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22351, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from Charles River.
IHC image of CD43 staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab22351 (shown in red) at 1µg/ml and co-localization of T cells stained using ab16669 (shown in green), Rabbit monoclonal to CD3, at 1/150 dilution. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®647, 1/1000)) and ab150077 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®488, 1/1000)) for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
Lewis rat splenocytes stained with ab22351 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22351) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab22351 has been referenced in 4 publications.
- De Miguel C et al. High dietary protein exacerbates hypertension and renal damage in Dahl SS rats by increasing infiltrating immune cells in the kidney. Hypertension 57:269-74 (2011). IHC-P ; Rat . PubMed: 21173345
- Skoumal R et al. Parthenolide inhibits STAT3 signaling and attenuates angiotensin II-induced left ventricular hypertrophy via modulation of fibroblast activity. J Mol Cell Cardiol 50:634-41 (2011). IHC-P ; Rat . PubMed: 21223972
- De Miguel C et al. Infiltrating T lymphocytes in the kidney increase oxidative stress and participate in the development of hypertension and renal disease. Am J Physiol Renal Physiol 300:F734-42 (2011). IHC-P ; Rat . PubMed: 21159736
- Moilanen AM et al. Intramyocardial BNP gene delivery improves cardiac function through distinct context-dependent mechanisms. Circ Heart Fail 4:483-95 (2011). PubMed: 21558448