Product nameCD43 overexpression 293T lysate (whole cell)
ab94089 is a 293T cell transfected lysate in which Human CD43 has been transiently over-expressed using a pCMV-CD43 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Tissue specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. PTM: Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
Our Abpromise guarantee covers the use of ab94089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94089 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-CD43 antibody (ab68421) at 1/500 dilution
Lane 1 :
CD43 overexpression 293T lysate (whole cell) (ab94089)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse
IgG (H and L) HRP conjugated at 1/2500 dilution
ab94089 has not yet been referenced specifically in any publications.