Anti-CD44 antibody [156-3C11] - BSA and Azide free (ab213072)

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Overview

  • Product name

    Anti-CD44 antibody [156-3C11] - BSA and Azide free
    See all CD44 primary antibodies

  • Description

    Mouse monoclonal [156-3C11] to CD44 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, Functional Studies, WBmore details

  • Species reactivity

    Reacts with: Human, Baboon, African green monkey

  • Immunogen

    Tissue, cells or virus corresponding to Human CD44. (Stimulated Human leukocytes).

  • Positive control

    • Human tonsil and breast carcinoma tissues; HeLa cells; Paracortex in tonsil or lymph node.

Properties

Applications

Our Abpromise guarantee covers the use of ab213072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).
Functional Studies Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 81 kDa.

Target

  • Function

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

  • Tissue specificity

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

  • Sequence similarities

    Contains 1 Link domain.

  • Domain

    The lectin-like LINK domain is responsible for hyaluronan binding.

  • Post-translational
    modifications

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • CDW44 antigen antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • HUTCHI antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH I antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] - BSA and Azide free (ab213072)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] - BSA and Azide free (ab213072)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling CD44 with ab213072 at 0.5 µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] - BSA and Azide free (ab213072)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] - BSA and Azide free (ab213072)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD44 with ab213072 at 0.5 µg/ml.

    References

    ab213072 has not yet been referenced specifically in any publications.

    Publishing research using ab213072? Please let us know so that we can cite the reference in this datasheet.

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