Recombinant Anti-CD44 antibody [1M7.8.1] (ab119348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1M7.8.1] to CD44
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD44 antibody [1M7.8.1]
See all CD44 primary antibodies -
Description
Rat monoclonal [1M7.8.1] to CD44 -
Host species
Rat -
Specificity
Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms. -
Tested applications
Suitable for: ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length protein corresponding to Mouse CD44.
Database link: P15379 -
Positive control
- ICC/IF: HeLa and NIH/3T3 cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 02nd November 2020
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1M7.8.1 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/250.
|
Notes |
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ICC/IF
1/250. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
- Omim: 107269 Human
- SwissProt: P16070 Human
- SwissProt: P15379 Mouse
- Unigene: 502328 Human
- Unigene: 423621 Mouse
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells. -
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab119348 has been referenced in 20 publications.
- Yamasaki S et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. iScience 25:103657 (2022). PubMed: 35024589
- Kashirina A et al. FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells. Cells 11:N/A (2022). PubMed: 36078136
- Carter LE et al. Transcriptional heterogeneity of stemness phenotypes in the ovarian epithelium. Commun Biol 4:527 (2021). PubMed: 33953351
- Huang X et al. Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds. Exp Ther Med 20:19 (2020). PubMed: 32934684
- Hu S et al. CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin. Cancer Cell Int 20:563 (2020). PubMed: 33292278