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    cd44-antibody-ab157107.pdf

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Immunology Cell Type Markers CD Adhesion
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Anti-CD44 antibody (ab157107)

  • Datasheet
  • SDS
Reviews (26) Submit a question References (93)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
  • Western blot - Anti-CD44 antibody (ab157107)
  • Western blot - Anti-CD44 antibody (ab157107)
  • Western blot - Anti-CD44 antibody (ab157107)
  • Western blot - Anti-CD44 antibody (ab157107)
  • Western blot - Anti-CD44 antibody (ab157107)
  • Immunoprecipitation - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)

Key features and details

  • Rabbit polyclonal to CD44
  • Suitable for: ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CD44 antibody [EPR18668] (ab189524)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CD44 antibody
    See all CD44 primary antibodies
  • Description

    Rabbit polyclonal to CD44
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 692-742 of Human CD44 (NP_000601.3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human skin tissue, pancreas cancer tissue, cervical cancer tissue, ovarian carcinoma tissue. colon carcinoma tissue; mouse spleen tissue; rat spleen tissue. ICC: C6 cell, MDA-MB-231 cell, MCF-7 cells, NIH/3T3 cells. WB:: MCF-7, Jurkat, MDA-MB-231, HeLa, NIH/3T3, HeLa, 293T, Jurkat, Raw264.7 cell lysate; Mouse brain and spleen tissue lysates; Human breast cancer, lung tissue and spleen tissue. IP: HeLa cells.
  • General notes

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor immunology
    • CD markers
    • Cancer
    • Tumor biomarkers
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human CD44 protein (Active) (ab173996)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Rat
Human
IHC-P
Mouse
Rat
Human
IP
Human
WB
Mouse
Human
All applications
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Baboon
Cynomolgus monkey
Rhesus monkey
Gorilla
Orangutan
Platypus
Application Abreviews Notes
ICC/IF (4)
Use at an assay dependent concentration.
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP
Use at 2-10 µg/mg of lysate.
IHC-P (10)
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP
Use at 2-10 µg/mg of lysate.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities

    Contains 1 Link domain.
  • Domain

    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P16070 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101008690 Baboon
    • Entrez Gene: 451122 Chimpanzee
    • Entrez Gene: 281057 Cow
    • Entrez Gene: 101867504 Cynomolgus monkey
    • Entrez Gene: 403939 Dog
    • Entrez Gene: 100034221 Horse
    • Entrez Gene: 960 Human
    • Entrez Gene: 12505 Mouse
    • Entrez Gene: 100525150 Pig
    • Entrez Gene: 25406 Rat
    • Entrez Gene: 717204 Rhesus monkey
    • Omim: 107269 Human
    • SwissProt: Q29423 Cow
    • SwissProt: Q28284 Dog
    • SwissProt: Q05078 Horse
    • SwissProt: P16070 Human
    • SwissProt: P15379 Mouse
    • SwissProt: P26051 Rat
    • Unigene: 502328 Human
    • Unigene: 423621 Mouse
    • Unigene: 1120 Rat
    see all
  • Alternative names

    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • CDW44 antigen antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • HUTCHI antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH I antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)

    ab157107 staining CD44 in C6 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilised with 0.1% Triton-X and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • Western blot - Anti-CD44 antibody (ab157107)
    Western blot - Anti-CD44 antibody (ab157107)
    All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : MCF-7 (human breast adenocarcinoma epithelial) whole cell lysates
    Lane 2 : Jurkat (human acute T cell leukaemia lymphocyte) whole cell lysates
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysates
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

  • Western blot - Anti-CD44 antibody (ab157107)
    Western blot - Anti-CD44 antibody (ab157107)
    All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    All lanes :

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to GNAT2 (ab97501) at 1/20000 dilution

    Predicted band size: 81 kDa


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • Western blot - Anti-CD44 antibody (ab157107)
    Western blot - Anti-CD44 antibody (ab157107)
    All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast) whole cell lysates
    Lane 2 : Raw264.7 (mouse macrophage) whole cell lysates
    Lane 3 : Mouse brain tissue lysates
    Lane 4 : Mouse spleen tissue lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • Western blot - Anti-CD44 antibody (ab157107)
    Western blot - Anti-CD44 antibody (ab157107)
    All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : Human breast cancer tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

     

  • Western blot - Anti-CD44 antibody (ab157107)
    Western blot - Anti-CD44 antibody (ab157107)
    All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-CD44 antibody (ab157107)
    Immunoprecipitation - Anti-CD44 antibody (ab157107)
    Detection of CD44 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157107(Lane 1). For WB detection an ab157107 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)

    ab157107 staining CD44 in MDA-MB-231 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)

    ab157107 showing negative staining of CD44 in MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)

    ab157107 showing weak staining of CD44 in NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (93)

Publishing research using ab157107? Please let us know so that we can cite the reference in this datasheet.

ab157107 has been referenced in 93 publications.

  • Takasugi M  et al. Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties. Nat Commun 11:2376 (2020). WB, IP . PubMed: 32398747
  • Fang M  et al. Abnormal CD44 activation of hepatocytes with nonalcoholic fatty accumulation in rat hepatocarcinogenesis. World J Gastrointest Oncol 12:66-76 (2020). PubMed: 31966914
  • Ge W  et al. ZGRF1 Is Associated with Poor Prognosis in Triple-Negative Breast Cancer and Promotes Cancer Stemness Based on Bioinformatics. Onco Targets Ther 13:2843-2854 (2020). PubMed: 32308418
  • Yoon JY  et al. Stem/progenitor cell marker expression in clear cell renal cell carcinoma: a potential relationship with the immune microenvironment to be explored. BMC Cancer 20:272 (2020). PubMed: 32245446
  • Li R  et al. Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization. Arthritis Res Ther 22:75 (2020). PubMed: 32272965
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 26 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 17 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Keratinocytes)
Permeabilization
No
Specification
Keratinocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Jan 21 2021

Immunohistochemistry (Frozen sections) abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Psoriasis)
Permeabilization
No
Specification
Psoriasis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Dec 21 2020

Western blot abreview for Anti-CD44 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. Dimitra Kalamida

Verified customer

Submitted Dec 25 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More

Abcam user community

Verified customer

Submitted Aug 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
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Abcam user community

Verified customer

Submitted Aug 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (skin)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 28 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: cirtric acid
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 27 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC3 Prostate cancer cells)
Permeabilization
Yes - 0.1% Triton X-100
Specification
PC3 Prostate cancer cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 21 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD44 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin, lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Skin, lung
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5%
Fixative
Formaldehyde
Read More

Mr. Victor Bernal

Verified customer

Submitted Oct 03 2018

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