Anti-CD44 antibody (ab157107)
Key features and details
- Rabbit polyclonal to CD44
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD44 antibody
See all CD44 primary antibodies -
Description
Rabbit polyclonal to CD44 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide within Human CD44 aa 650-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P16070-1 -
Positive control
- IHC-P: Human squamous cell carcinoma, lung cancer, skin, cervical cancer. Mouse spleen and plasmacytoma tissue; rat spleen tissue. WB: A549, HeLa, U2OS, TCMK-1, NIH/3T3 cells IP: HeLa and HAP1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P | (11) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 451122 Chimpanzee
- Entrez Gene: 281057 Cow
- Entrez Gene: 101867504 Cynomolgus monkey
- Entrez Gene: 403939 Dog
- Entrez Gene: 100034221 Horse
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
see all -
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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Western blot - Anti-CD44 antibody (ab157107)
ab157107 was shown to react with CD44 in wild-type HAP1 cells in Western blot with loss of signal observed in a CD44 knockout cell line. Wild-type HAP1 and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab157107 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of mouse plasmacytoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
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Immunoprecipitation - Anti-CD44 antibody (ab157107)Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines. -
Immunoprecipitation - Anti-CD44 antibody (ab157107)Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human lung cancer tissue staining CD-44 with ab157107 at 1:5000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human head and neck squamous cell carcinoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
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Immunoprecipitation - Anti-CD44 antibody (ab157107)Immunoprecipitatgion of HeLa cells with:
Lane 1: Rabbit anti-CD44 recombinant monoclonal antibody
Lane 2: ab157107 lot A303-872A-3
Lane 3: ab157107 lot A303-872A-4
Lane 4: Control IgG
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml
Lane 1 : TMCK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 81 kDa
Exposure time: 30 seconds -
Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 0.04 µg/ml
Lane 1 : A-549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : U20S (human bone osteosarcoma epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 81 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (143)
ab157107 has been referenced in 143 publications.
- Liu Z et al. Secreted TRAIL gene-modified adipose-derived stem cells exhibited potent tumor-suppressive effect in hepatocellular carcinoma cells. Immun Inflamm Dis 9:144-156 (2021). PubMed: 33156578
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Wang Z et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370
- Zhang W et al. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner. Cell Death Dis 12:102 (2021). PubMed: 33473125
- Yeung CY et al. Immune Modulation Effects of Lactobacillus casei Variety rhamnosus on Enterocytes and Intestinal Stem Cells in a 5-FU-Induced Mucositis Mouse Model. Gastroenterol Res Pract 2021:3068393 (2021). PubMed: 33564301