Anti-CD44 antibody (ab157107)
- Datasheet
- References (34)
- Protocols
Overview
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Product nameAnti-CD44 antibody
See all CD44 primary antibodies -
DescriptionRabbit polyclonal to CD44
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 692-742 of Human CD44 (NP_000601.3).
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Positive control
- HeLa, 293T and Jurkat whole cell lysates. IF/ICC: HeLa cell line
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 81 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Sequence similaritiesContains 1 Link domain.
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DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
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Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 451122 Chimpanzee
- Entrez Gene: 281057 Cow
- Entrez Gene: 101867504 Cynomolgus monkey
- Entrez Gene: 403939 Dog
- Entrez Gene: 100034221 Horse
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
see all -
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Flow Cytometry - Anti-CD44 antibody (ab157107)This image is courtesy of an anonymous Abreviewab157107 staining CD44 in the Human gastric cancer cell line by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. The sample was incubated with the primary antibody (1/100) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Gating Strategy: Live Cells. -
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 staining CD44 in C6 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilised with 0.1% Triton-X and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : MCF-7 (human breast adenocarcinoma epithelial) whole cell lysates
Lane 2 : Jurkat (human acute T cell leukaemia lymphocyte) whole cell lysates
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysates
Lane 4 : HeLa (human cervix adenocarcinoma epithelial) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
All lanes :
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to GNAT2 (ab97501) at 1/20000 dilution
Predicted band size: 81 kDa
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST
Diluting Buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast) whole cell lysates
Lane 2 : Raw264.7 (mouse macrophage) whole cell lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse spleen tissue lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteBlocking Buffer: 5% NFDM/TBST
Diluting Buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : Human breast cancer tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CD44 antibody (ab157107)Detection of CD44 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157107(Lane 1). For WB detection an ab157107 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 staining CD44 in MDA-MB-231 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 showing negative staining of CD44 in MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 showing weak staining of CD44 in NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
Protocols
References
This product has been referenced in:
- Tang YC et al. Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo. Int J Oncol 52:127-138 (2018). Read more (PubMed: 29115601) »
- Connolly NP et al. Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma. Sci Rep 8:1180 (2018). Read more (PubMed: 29352201) »
