Anti-CD44 antibody (ab157107)
Key features and details
- Rabbit polyclonal to CD44
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD44 antibody
See all CD44 primary antibodies -
Description
Rabbit polyclonal to CD44 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseRatHumanIHC-P MouseRatHumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 692-742 of Human CD44 (NP_000601.3).
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Positive control
- IHC-P: Human skin tissue, pancreas cancer tissue, cervical cancer tissue, ovarian carcinoma tissue. colon carcinoma tissue; mouse spleen tissue; rat spleen tissue. ICC: C6 cell, MDA-MB-231 cell, MCF-7 cells, NIH/3T3 cells. WB:: MCF-7, Jurkat, MDA-MB-231, HeLa, NIH/3T3, HeLa, 293T, Jurkat, Raw264.7 cell lysate; Mouse brain and spleen tissue lysates; Human breast cancer, lung tissue and spleen tissue. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
Rat
Human
|
| IHC-P |
Mouse
Rat
Human
|
| IP |
Human
|
| WB |
Mouse
Human
|
| All applications |
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Baboon
Cynomolgus monkey
Rhesus monkey
Gorilla
Orangutan
Platypus
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (4) |
Use at an assay dependent concentration.
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| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P | (10) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 451122 Chimpanzee
- Entrez Gene: 281057 Cow
- Entrez Gene: 101867504 Cynomolgus monkey
- Entrez Gene: 403939 Dog
- Entrez Gene: 100034221 Horse
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
see all -
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 staining CD44 in C6 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilised with 0.1% Triton-X and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : MCF-7 (human breast adenocarcinoma epithelial) whole cell lysates
Lane 2 : Jurkat (human acute T cell leukaemia lymphocyte) whole cell lysates
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysates
Lane 4 : HeLa (human cervix adenocarcinoma epithelial) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
All lanes :
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to GNAT2 (ab97501) at 1/20000 dilution
Predicted band size: 81 kDa
Exposure time: 10 secondsBlocking buffer: 5% NFDM/TBST
Diluting Buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast) whole cell lysates
Lane 2 : Raw264.7 (mouse macrophage) whole cell lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Mouse spleen tissue lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteBlocking Buffer: 5% NFDM/TBST
Diluting Buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution
Lane 1 : Human breast cancer tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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Western blot - Anti-CD44 antibody (ab157107)All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CD44 antibody (ab157107)Detection of CD44 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157107(Lane 1). For WB detection an ab157107 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 staining CD44 in MDA-MB-231 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
-
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 showing negative staining of CD44 in MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
-
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody (ab157107)ab157107 showing weak staining of CD44 in NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.
Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.
Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (93)
ab157107 has been referenced in 93 publications.
- Takasugi M et al. Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties. Nat Commun 11:2376 (2020). WB, IP . PubMed: 32398747
- Fang M et al. Abnormal CD44 activation of hepatocytes with nonalcoholic fatty accumulation in rat hepatocarcinogenesis. World J Gastrointest Oncol 12:66-76 (2020). PubMed: 31966914
- Ge W et al. ZGRF1 Is Associated with Poor Prognosis in Triple-Negative Breast Cancer and Promotes Cancer Stemness Based on Bioinformatics. Onco Targets Ther 13:2843-2854 (2020). PubMed: 32308418
- Yoon JY et al. Stem/progenitor cell marker expression in clear cell renal cell carcinoma: a potential relationship with the immune microenvironment to be explored. BMC Cancer 20:272 (2020). PubMed: 32245446
- Li R et al. Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization. Arthritis Res Ther 22:75 (2020). PubMed: 32272965
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