Overview

  • Product name
  • Description
    Rabbit polyclonal to CD44
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 692-742 of Human CD44 (NP_000601.3).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates. IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab157107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
IP Use at 2-10 µg/mg of lysate.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities
    Contains 1 Link domain.
  • Domain
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • ab157107 staining CD44 in the Human gastric cancer cell line by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. The sample was incubated with the primary antibody (1/100) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: Live Cells.

     

    See Abreview

  • ab157107 staining CD44 in C6 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilised with 0.1% Triton-X and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : MCF-7 (human breast adenocarcinoma epithelial) whole cell lysates
    Lane 2 : Jurkat (human acute T cell leukaemia lymphocyte) whole cell lysates
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysates
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

    The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

  • All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    All lanes :

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to GNAT2 (ab97501) at 1/20000 dilution

    Predicted band size: 81 kDa


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast) whole cell lysates
    Lane 2 : Raw264.7 (mouse macrophage) whole cell lysates
    Lane 3 : Mouse brain tissue lysates
    Lane 4 : Mouse spleen tissue lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    Blocking Buffer: 5% NFDM/TBST

    Diluting Buffer: 5% NFDM/TBST

  • All lanes : Anti-CD44 antibody (ab157107) at 1/10000 dilution

    Lane 1 : Human breast cancer tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Additional bands at: 81 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

     

  • All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 3 minutes
  • Detection of CD44 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157107(Lane 1). For WB detection an ab157107 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab157107 at 1/2000 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CD44 with ab157107 at 1/1000 (1µg/ml). Detection: DAB.
  • ab157107 staining CD44 in MDA-MB-231 cell membranes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • ab157107 showing negative staining of CD44 in MCF-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody, both at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

  • ab157107 showing weak staining of CD44 in NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody (1/1000). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) was used as the secondary antibody at a dilution of 1/1000, shown in the top left hand panel. Additionally, ab7291 anti-tubulin and nuclear stain DAPI (blue) were used as counterstains as shown in the top right hand panel.

    Negative control 1: Primary ab157107 and ab150120 Alexa Fluor®594 goat anti-mouse secondary were both used at a dilution of 1/1000.

    Negative control 2: ab7291 anti-tubulin and ab150077 AlexaFluor®488 Goat anti-Rabbit secondary were both used at a dilution of 1/1000.

References

This product has been referenced in:
  • Tang YC  et al. Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo. Int J Oncol 52:127-138 (2018). Read more (PubMed: 29115601) »
  • Connolly NP  et al. Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma. Sci Rep 8:1180 (2018). Read more (PubMed: 29352201) »
See all 34 Publications for this product

Customer reviews and Q&As

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1-10 of 17 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin, lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Skin, lung
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5%
Fixative
Formaldehyde

Victor Bernal

Verified customer

Submitted Oct 03 2018

Application
Flow Cytometry
Sample
Rabbit Cell (adipose)
Permeabilization
No
Specification
adipose
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 05 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jan 19 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jan 19 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Jan 19 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cat Cell lysate - whole cell (Skin Fibroblasts and Vaccination Associated Sarcom)
Gel Running Conditions
Reduced Denaturing (12% Gel)
Loading amount
10 µg
Specification
Skin Fibroblasts and Vaccination Associated Sarcom
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Oct 20 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (mesenchymal stem cell from umbilical cord)
Permeabilization
Yes - 0.5% triton
Specification
mesenchymal stem cell from umbilical cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Myah Goldstein

Verified customer

Submitted Oct 02 2017

Application
Flow Cytometry
Sample
Rabbit Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 12 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - Cold methanol 90%
Gating Strategy
Living cells
Specification
Olfactory stem cells
Preparation
Cell harvesting/tissue preparation method: Cultured stem cells
Sample buffer: 10% FBS in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2017

1-10 of 17 Abreviews

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