Overview

  • Product name
  • Description
    Rabbit polyclonal to CD44
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CD44.
    (Peptide available as ab52122)

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab24504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IP
  • Application notes
    IHC-Fr: Use at a concentration of 20 µg/ml.
    IP: Use at a concentration of 20 µg/ml.
    WB: Use at a concentration of 1-4 µg/ml. Predicted molecular weight: 82 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificity
      Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similarities
      Contains 1 Link domain.
    • Domain
      The lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    Images

    • All lanes : Anti-CD44 antibody (ab24504) at 2 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 82 kDa
      Observed band size: 82 kDa
      Additional bands at: 25-50 kDa (possible isoform)

    • Immunohistochemical analysis of Human prostate cancer cell xenograft tissue, staining CD44 (red) with ab24504.

      Tissue was fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% serum. Samples were incubated overnight with primary antibody (1/100) overnight at 4°C. An AlexaFluor®555-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

    References

    This product has been referenced in:
    • Hu Z  et al. Transient receptor potential vanilloid-type 2 targeting on stemness in liver cancer. Biomed Pharmacother 105:697-706 (2018). Read more (PubMed: 29906748) »
    • Mayr L  et al. CD44 drives aggressiveness and chemoresistance of a metastatic human osteosarcoma xenograft model. Oncotarget 8:114095-114108 (2017). Read more (PubMed: 29371972) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us.

    The link actually does not show results, I am sorry for the inconvenience.

    Alternatively you can type CD44 in the search box provided on the website. The system will automatically give you sorted anti CD44 antibodies. Please select the antibodies based on your requirements of clonality, immunogen, species and application.

    The most common are ab119863, ab6124, ab51037, ab19622, ab65829, ab25340, ab24504, ab119348 and ab25579 etc.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for your enquiry. At this point we do not have any data from testing the different isoform specificity of this antibody. All of our products are guaranteed to work in species and application as stated on the datasheet. Should you not get expected results, then please inform us within the guarantee period of 6 month (Abpromise). We will help troubleshoot the experiment and if the product is indeed faulty, we will be more than happy to offer a free replacement or refund. I hope our Abpromise guarantee will give you the assurance you need to use this product in your research.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (lung)
    Specification
    lung
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Mr. Pete Geisen

    Verified customer

    Submitted Jan 07 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (retina)
    Specification
    retina
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Mr. Pete Geisen

    Verified customer

    Submitted Jan 07 2008

    Question
    Answer

    Thank you for your enquiry. HeLa cell extract can be used as a positive control with this antibody. Please contact us again if you have any additional questions.

    Read More

    Answer

    Thank you for getting back to me with an image of your blot. Once again I am sorry to hear that you have been having difficulties with this antibody. Your image is very convincing and the fact that you are not observing a band of 82KDa from a murine lysate is very telling. This is despite the prolonged exposure of your blot. Indeed the large number of extraneous bands that you have obtained in the embryo lysate is of some concern. Quality is important to Abcam. Given that this antibody has not behaved as detailed on our datasheet against your mouse lysates, I would like to offer you a credit note provided that the antibody was purchased within the past 90 days. For me to do this please can you e-mail me details of the date of purchase and original purchase order number. I look forward to hearing from you.

    Read More

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody by IP. I noticed that you have also made an enquiry about this antibody by western blotting. Quality is important to Abcam. I would appreciate it if you could provide me with the details that I mentioned in my previous e-mail regarding further information of the conditions that you have been using by western blotting. Should this antibody not work by western, I consider it possible that it will not work by immunoprecipitation. With regards your IP conditions, please can you tell me how much antibody that you have been using. This antibody is supplied quite dilute (0.2mgml-1) with a recommended dilution of 20ugml-1 by IP; therefore a 1:10 dilution is required. I would also be interested in the wash conditions that you have been using and whether you could quantitate the mass of protein that you have been using. We recommend 200ug - 500ug RIPA lysate. I appreciate your patience in this matter and look forward to your response.

    Read More

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your questionnaire and I have a few comments. It would greatly help me if you could provide me with a quantitation value of the mass of protein that you are loading. I would like to recommend that you quantitate the protein and load 20-40ug for analysis by western blotting. It would also assist me if you could provide me with details of how the samples were prepared for electrophoresis. Did you use denaturing (SDS) conditions in addition to reduced conditions? Were the samples prepared using Laemmli buffer? Did you verify the integrity and successful transfer of the protein by using a Ponceau stain of the membrane? Also could you please provide me with details of the secondary antibodies you have used. Finally I would appreciate it if you could attach an image of the blot with the molecular weight markers marked.

    Read More

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