Recombinant Anti-CD44 antibody [BLR038F] (ab243894)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-334369-anti-cd44-antibody-blr038f-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR038F] to CD44
- Suitable for: Flow Cyt, ICC, IHC-P, IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CD44 antibody [BLR038F]
See all CD44 primary antibodies -
Description
Rabbit monoclonal [BLR038F] to CD44 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD44 aa 700 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_000601.3 and Gene ID 960.
Database link: P16070-1 -
Positive control
- WB: 786-O, KG-1, HeLa and A549 cell lysates. IHC-P: Human head and neck squamous cell carcinoma tissue. ICC: HCT116 cells. Flow Cyt: HeLa cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR038F -
Isotype
IgG -
Research areas
Associated products
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µl for 106 cells.
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| ICC |
1/100 - 1/500.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
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| WB |
1/1000.
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| Notes |
|---|
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Flow Cyt
Use 1µl for 106 cells. |
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ICC
1/100 - 1/500. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
|
WB
1/1000. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)Formalin-fixed, paraffin-embedded human head and neck squamous cell carcinoma tissue stained for CD44 using ab243894 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-400170-anti-cd44-antibody-blr038f-western-blot-hela-wt-hela-cd44-knockout-a549-lncap.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)All lanes : Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Knockout CD44 HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : LnCAP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab243894 observed at 80 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab243894 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab243894 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-334372-anti-cd44-antibody-blr038f-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)CD44 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243894 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243894 at 1/1000 dilution.Lane 1: ab243894 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
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![Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-464306-anti-cd44-antibody-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)Flow Cytometry analysis of HeLa cells labeling CD44 with ab243894 (red). Cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG. -
![Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-334370-anti-cd44-antibody-blr038f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling CD44 using ab243894 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining. -
![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-334371-anti-cd44-antibody-blr038f-western-blot.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)Western blot analysis using ab243894 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: 786-O whole cell lysate (50 µg).
Lane 3: HEK-293T whole cell lysate (50 µg).
Lane 4: Jurkat-3 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: MCF7 whole cell lysate (50 µg).
Lane 7: HepG2 whole cell lysate (50 µg).
Lane 8: KG-1 whole cell lysate (50 µg).
Lane 9: RPMI 8226 whole cell lysate (50 µg).
Lane 10: MOLT-4 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
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![Western blot - Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-464310-anti-cd44-antibody-wester-blot-el4-mouse.jpg)
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)All lanes : Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1 : BW5147.3 cell lysate
Lane 2 : EL4 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 10 secondsDetection method: Chemiluminescence
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![Anti-CD44 antibody [BLR038F] (ab243894)](https://www.abcam.com/ps/products/243/ab243894/Images/ab243894-6-benefits-of-recombinant-antibodies.png)
Anti-CD44 antibody [BLR038F] (ab243894)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243894 has been referenced in 1 publication.
- Li S et al. Schisandrin B inhibits epithelial-mesenchymal transition and stemness of large-cell lung cancer cells and tumorigenesis in xenografts via inhibiting the NF-?B and p38 MAPK signaling pathways. Oncol Rep 45:N/A (2021). PubMed: 33907830