Product nameAnti-CD44 antibody [EPR1013Y]
See all CD44 primary antibodies
DescriptionRabbit monoclonal [EPR1013Y] to CD44
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human CD44 aa 150-250. The exact sequence is proprietary.
- Recombinant Human CD44 protein (ab114154) can be used as a positive control in WB. TF-1 cell lysate or human urinary bladder tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 3.76 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 488) (ab194987)
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 647) (ab194988)
- Anti-CD44 antibody [EPR1013Y] (HRP) (ab194989)
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 594) (ab201724)
- Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)
- Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
Our Abpromise guarantee covers the use of ab51037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
- Information by UniProt
- LHR antibody
- BA-1 antibody
- CD 44 antibody
ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/1000 dilution
Lane 1 : MCF-7 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : MDA-MB-231 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and diluting buffer 5% NFDM/TBST
The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).
ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
ab51037 (1:100) showing positive staining in human Glioma tissue.
ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Gao Y et al. LXRa Promotes the Differentiation of Human Gastric Cancer Cells through Inactivation of Wnt/ß-catenin Signaling. J Cancer 10:156-167 (2019). Read more (PubMed: 30662536) »
- Zhang C et al. TGFß1 Promotes Breast Cancer Local Invasion and Liver Metastasis by Increasing the CD44high/CD24- Subpopulation. Technol Cancer Res Treat 17:1533033818764497 (2018). Read more (PubMed: 29658391) »