Overview

  • Product name
    Anti-CD44 antibody [EPR1013Y]
    See all CD44 primary antibodies
  • Description
    Rabbit monoclonal [EPR1013Y] to CD44
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD44 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • Recombinant Human CD44 protein (ab114154) can be used as a positive control in WB. TF-1 cell lysate or human urinary bladder tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificity
      Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similarities
      Contains 1 Link domain.
    • Domain
      The lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • INLU-related p80 Glycoprotein antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      • Soluble CD44 antibody
      see all

    Images

    • ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.

    • Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/1000 dilution

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : MDA-MB-231 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 82 kDa
      Observed band size: 81 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer 5% NFDM/TBST

      The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

    • ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size: 82 kDa
      Observed band size: 82 kDa

    • ab51037 (1:100) showing positive staining in human Glioma tissue.

    • ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Zhang C  et al. TGFß1 Promotes Breast Cancer Local Invasion and Liver Metastasis by Increasing the CD44high/CD24- Subpopulation. Technol Cancer Res Treat 17:1533033818764497 (2018). Read more (PubMed: 29658391) »
    • Azevedo R  et al. Glycan affinity magnetic nanoplatforms for urinary glycobiomarkers discovery in bladder cancer. Talanta 184:347-355 (2018). Read more (PubMed: 29674052) »
    See all 78 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-10 of 10 Abreviews

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2 liver cancer cell line)
    Permeabilization
    Yes - 0.1% Triton X100 in PBS
    Specification
    HepG2 liver cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Sep 21 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela)
    Gel Running Conditions
    Non-reduced Denaturing (7.5% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Hela
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 10 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample
    Mouse Tissue sections (Mouse 15.5dpc)
    Specification
    Mouse 15.5dpc
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 14 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Sample
    Mouse Cell (differentiating ES)
    Specification
    differentiating ES
    Gating Strategy
    live cells
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme free

    Abcam user community

    Verified customer

    Submitted Jul 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Formalin
    Antigen retrieval step
    Other
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Apr 12 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MB-MDA-231)
    Loading amount
    20 µg
    Specification
    MB-MDA-231
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

    Ms. Zahra Abdalla

    Verified customer

    Submitted Dec 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB-231)
    Loading amount
    100000 cells
    Specification
    MDA-MB-231
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 04 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (MDA-MB-231)
    Specification
    MDA-MB-231
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme free
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells

    Abcam user community

    Verified customer

    Submitted Jan 09 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    10000 cells
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up