Application
Western blot
Sample
Human Cell lysate - whole cell (MB-MDA-231)
Loading amount
20 µg
Specification
MB-MDA-231
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000
Detection
Detection method
ECL
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 82 kDa
Positive control
MB-MDA-231
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Zahra Abdalla
Verified customer
Submitted Dec 19 2012