Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (differentiating ES)
Specification
differentiating ES
Gating Strategy
live cells
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme free
Sample buffer: enzyme free
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: 0.2% BSA and 0.1% NaAz in PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200
Additional data
Additional Notes
Tested this antibody alongside ab112178 which definitely does work for Flow Cytometry on mESC and ab112178 gave a positive result for the cell population tested here. Purple = control (secondary only), green = ab51037.
Abcam response
Thank you for sharing this data with us. We do not expect ab51037 to cross react with mouse based on its immunogen sequence (<80% sequence homology with mouse). For customers looking for a monoclonal antibody to use in flow cytometry on mouse cells, Abcam would recommend ab112178 or ab40983.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jul 09 2013