Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Gel Running Conditions
Non-reduced Denaturing (7.5% SDS-PAGE)
Loading amount
40 µg
Specification
Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk dilution in TBST
Dilution
1/5000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 82 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Sep 10 2014