The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Contains 1 Link domain.
The lectin-like LINK domain is responsible for hyaluronan binding.
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
hematopoietic cell E- and L-selectin ligand antibody
Heparan sulfate proteoglycan antibody
Hermes antigen antibody
homing function and Indian blood group system antibody
Hyaluronate receptor antibody
INLU-related p80 Glycoprotein antibody
Phagocytic glycoprotein 1 antibody
Phagocytic glycoprotein I antibody
Soluble CD44 antibody
Western blot - Anti-CD44 antibody [EPR1013Y] (HRP) (ab194989)
Anti-CD44 antibody [EPR1013Y] (HRP) (ab194989) at 1/2500 dilution + TF-1 Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa Observed band size: 82 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194989 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.