Recombinant Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)
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Overview
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Product name
Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free
See all CD44 primary antibodies -
Description
Rabbit monoclonal [EPR1013Y] to CD44 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CD44 aa 150-250. The exact sequence is proprietary.
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Positive control
- TF-1 cell lysate or human urinary bladder tissue.
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General notes
ab246690 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 3.76 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Endotoxin level is less than 1 EU/ml as determined by the TAL test. -
Clonality
Monoclonal -
Clone number
EPR1013Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 488) (ab194987)
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 647) (ab194988)
- Anti-CD44 antibody [EPR1013Y] (HRP) (ab194989)
- Anti-CD44 antibody [EPR1013Y] (Alexa Fluor® 594) (ab201724)
- Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)
- Anti-CD44 antibody [EPR1013Y] (ab51037)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab246690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-16-ab51037265962Untitled3.jpg)
Flow Cytometry - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-14-ab5103725803851037humantonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-13-ab5103725803751037humanskin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-12-ab5103725803651037humanpancreascancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-11-ab5103725803551037humancervixcancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-9-CD44Primaryantibodiesab5103720.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-8-CD44Primaryantibodiesab5103721.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)ab51037 (1:100) showing positive staining in human Glioma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-7-CD44Primaryantibodiesab5103722.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Other - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)](https://www.abcam.com/ps/products/246/ab246690/Images/ab246690-6-CD44Primaryantibodiesab5103749.jpg)
Other - Anti-CD44 antibody [EPR1013Y] - Low endotoxin, Azide free (ab246690)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51037).
Datasheets and documents
References
ab246690 has not yet been referenced specifically in any publications.
