• Product name
    Anti-CD44 antibody [F10-44-2] (PE/Cy7 ®)
    See all CD44 primary antibodies
  • Description
    Mouse monoclonal [F10-44-2] to CD44 (PE/Cy7 ®)
  • Host species
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human T cells

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.




Our Abpromise guarantee covers the use of ab46793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. See Abreview.
Flow Cyt 1/10 - 1/100.

ab103534 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • Function
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities
    Contains 1 Link domain.
  • Domain
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all


  • ab46793 staining CD44 in Human pancreatic adenocarcinoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody 1/200 (1% BSA in PBS)  for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/300, was used as secondary antibody.

    See Abreview

  • Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD44 with ab46793 at 10 μL/106 cells (purple). Grey - Isotype Control, Mouse IgG2a-PE/CY7.

  • Overlay histogram showing peripheral blood lymphocytes stained with ab46793 (red line). The cells were incubated with the antibody (ab46793, 1/100) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.


This product has been referenced in:
  • Tsuji W  et al. An Animal Model of Local Breast Cancer Recurrence in the Setting of Autologous Fat Grafting for Breast Reconstruction. Stem Cells Transl Med 7:125-134 (2018). Read more (PubMed: 29283514) »
  • Smeriglio P  et al. Soluble Collagen VI treatment enhances mesenchymal stem cells expansion for engineering cartilage. Bioeng Transl Med 2:278-284 (2017). Read more (PubMed: 29313037) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Pancreatic adenocarcinoma)
Pancreatic adenocarcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Jennifer Dembinski

Verified customer

Submitted Nov 17 2009


Vielen Dank für die Anfrage. Dass ab46793 nicht auf der Liste auftaucht, bedeutet dass er nicht getestet wurde, ob er mit Maus kreuzreagiert. In der Tat ist ab46793 für die Immunhistochemie (IHC-P/Fr - neben anderen Anwendungen wie Durchflusszytometrie) garantiert. Allerdings ist dieser CD44- Antikörper mit dem Tandemkonjugat PE-Cy7 gekoppelt. Welche Anregungswellenlängen beziehungsweise Filter Du für die Detektion benötigst, kannst Du anhand des Spektrums ersehen (Details dazu in Spectra-Viewern von eBioscience http://www.ebioscience.com/resources/fluorplan-spectra-viewer.htm  oder Biolegend http://www.biolegend.com/spectraanalyzer etc.) und beim Hersteller des Mikroskops erfragen. Im Allgemeinen sind PE und Tandem-Fluorochrome wie PE-Cy7 jedoch recht instabil und besitzen eine schwache Fluoreszenz-Intensität, so dass sie in der Regel wenig für die Mikroskopie geeignet sind. Wie auf dem Datenblatt von ab46793 beschrieben, würde ich deswegen für die Detektion dieses CD44- Antikörpers in der IHC einen sekundären Antikörper wie zum Beispiel ab96879 oder ab98697 empfehlen (je nach Fluorochrom-Belegung bei einer Mehrfachfärbung beziehungsweise vorhandenem Filtersystem). Daher ist es eigentlich nicht notwendig, den Klon [F10-44-2] als PE-Cy7-Konjugat zu nehmen (es sei denn, es sind noch andere Experimente damit geplant), sondern Du kannst ihn auch unkonjugiert erwerben (ab6124): ab6124: Click here (or use the following: https://www.abcam.com/index.html?datasheet=6124). ab96879: Click here (or use the following: https://www.abcam.com/index.html?datasheet=96879). ab98697: Click here (or use the following: https://www.abcam.com/index.html?datasheet=98697). Ich hoffe, diese Informationen helfen Dir weiter. Bitte zögere nicht, Dich wieder bei uns zu melden, falls Du weitere Fragen hast. 

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Dein Suchergebnis kann ich leider bestätigen: Wir haben derzeit keinen anti-human-nicht-Maus-kreuzreaktiven CD44- Antikörper erhältlich. Tut mir leid, dass ich keine besseren Nachrichten habe. Für weitere Fragen stehe ich jederzeit gerne zur Verfügung.

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