Overview

  • Product name
    Anti-CD44 antibody [KM201]
    See all CD44 primary antibodies
  • Description
    Rat monoclonal [KM201] to CD44
  • Host species
    Rat
  • Tested applications
    Suitable for: IP, WB, Flow Cyt, IHC-FoFr, Blocking, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitope
    ab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
  • Positive control
    • Flow Cyt: BALB/c mouse bone marrow cells.
  • General notes

    ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 85 kDa.
Flow Cyt Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 22654110
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100.

Target

  • Function
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities
    Contains 1 Link domain.
  • Domain
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all

Images

  • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25340 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG1-FITC was used as the secondary antibody. Grey - Isotype Control, Rat IgG1-UNLB, followed by Mouse Anti-Rat IgG1-FITC.

  • Effect of CD44 blockade on hematopoiesis.
    Long term bone marrow cultures from Has1/2/3 triple knock-out mice were treated with 300 µM 4MU or vehicle control during weekly feedings. Where indicated, 100 µg/ml HMW HA (UC, 600-1,500 kD) or 10 µg/ml CD44-specific antibody (ab25340) were added.
  • ab25340 staining CD44 in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with the primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Lim HY  et al. Hyaluronan Receptor LYVE-1-Expressing Macrophages Maintain Arterial Tone through Hyaluronan-Mediated Regulation of Smooth Muscle Cell Collagen. Immunity 49:326-341.e7 (2018). Read more (PubMed: 30054204) »
  • Inubushi T  et al. Aberrant perichondrial BMP signaling mediates multiple osteochondromagenesis in mice. JCI Insight 2:N/A (2017). Read more (PubMed: 28768899) »
See all 17 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 08 2014

Answer

DISCOUNT CODE: *******
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab25340 in Human and IHC-Fr. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forHuman and/or IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us again.

I am sorry I have over-read your main question. We state only mouse as a tested species on the datasheet so far. All tested applicationsare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.Therefore, it is unlikely that this antibody has been tested on human tissue so far.

However, I performed a BLAST search in which I compared the mouse CD44 Isoform 1 with the human protein and most of the human isoforms have a very high homology ( between 87 and 100%), therefore, I am positive that the antibody should detect in human tissue. Unfortunately, we cannot guarantee that it will work.

For the future, if you would like to test the antibody inan untested application or species, please contact our Scientific Support team prior to the purchase as you may be eligible for our testing discount program.

However, as the antibody has been already bought, we would like to encourage you to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

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Answer

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. This information is stated on the datasheet in the immunogen section.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

Should you have any further questions, please do not hesitate to contact us.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (T-cell lymphoma)
Specification
T-cell lymphoma
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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