- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-CD44 antibody [KM201]
See all CD44 primary antibodies -
DescriptionRat monoclonal [KM201] to CD44
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Host speciesRat
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Tested applicationsSuitable for: IP, WB, Flow Cyt, IHC-FoFr, Blocking, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Epitopeab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.
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Positive control
- Flow Cyt: BALB/c mouse bone marrow cells.
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General notes
ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone numberKM201
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab25340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 85 kDa. | |
| Flow Cyt | Use at an assay dependent concentration. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-FoFr | Use at an assay dependent concentration. | |
| Blocking | Use at an assay dependent concentration. PubMed: 22654110 | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/100. |
Target
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FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Sequence similaritiesContains 1 Link domain.
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DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
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Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 12505 Mouse
- SwissProt: P15379 Mouse
- Unigene: 423621 Mouse
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [KM201] (ab25340)](https://www.abcam.com/ps/products/25/ab25340/Images/ab25340-269898-ab25340FC.jpg)
Flow Cytometry - Anti-CD44 antibody [KM201] (ab25340)Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25340 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG1-FITC was used as the secondary antibody. Grey - Isotype Control, Rat IgG1-UNLB, followed by Mouse Anti-Rat IgG1-FITC.
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![Blocking - Anti-CD44 antibody [KM201] (ab25340)](https://www.abcam.com/ps/products/25/ab25340/Images/CD44-Primary-antibodies-ab25340-2.jpg)
Blocking - Anti-CD44 antibody [KM201] (ab25340)Image from Goncharova V et al., J Biol Chem. 2012 Jul 20;287(30):25419-33. Epub 2012 May 31.; Fig S3.; doi: 10.1074/jbc.M112.376699; July 20, 2012 The Journal of Biological Chemistry, 287, 25419-25433.Effect of CD44 blockade on hematopoiesis.
Long term bone marrow cultures from Has1/2/3 triple knock-out mice were treated with 300 µM 4MU or vehicle control during weekly feedings. Where indicated, 100 µg/ml HMW HA (UC, 600-1,500 kD) or 10 µg/ml CD44-specific antibody (ab25340) were added. -
![Immunohistochemistry (Frozen sections) - Anti-CD44 antibody [KM201] (ab25340)](https://www.abcam.com/ps/products/25/ab25340/Images/ab25340-276683-ab25340ihcfr.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD44 antibody [KM201] (ab25340)This image is courtesy of an anonymous Abreviewab25340 staining CD44 in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with the primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Trial J et al. Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis. Basic Res Cardiol 112:34 (2017). Mouse . Read more (PubMed: 28478479) »
- Inubushi T et al. Aberrant perichondrial BMP signaling mediates multiple osteochondromagenesis in mice. JCI Insight 2:N/A (2017). Read more (PubMed: 28768899) »
Customer reviews and Q&As
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