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The details of the immunogen for this antibody are not available.
ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.
Our Abpromise guarantee covers the use of ab25340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 85 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. PubMed: 22654110|
|IHC-P||Use at an assay dependent concentration.|
Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25340 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG1-FITC was used as the secondary antibody. Grey - Isotype Control, Rat IgG1-UNLB, followed by Mouse Anti-Rat IgG1-FITC.
ab25340 staining CD44 in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with the primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
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