Anti-CD44 antibody [KM201] (Phycoerythrin) (ab25224)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CD44 antibody [KM201] (Phycoerythrin)
See all CD44 primary antibodies -
Description
Rat monoclonal [KM201] to CD44 (Phycoerythrin) -
Host species
Rat -
Conjugation
Phycoerythrin. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
ab25224 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
ab25224 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells. -
Clonality
Monoclonal -
Clone number
KM201 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.2µg for 106 cells. ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 80 - 95 kDa. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12505 Mouse
- SwissProt: P15379 Mouse
- Unigene: 423621 Mouse
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [KM201] (Phycoerythrin) (ab25224)](https://www.abcam.com/ps/products/25/ab25224/Images/ab25224-269899-anti-cd44-antibody-km201-phycoerythrin-flow-cytometry.jpg)
Flow Cytometry - Anti-CD44 antibody [KM201] (Phycoerythrin) (ab25224)Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25224 (Phycoerythrin) at 0.1 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG1-Phycoerythrin.
Datasheets and documents
References
This product has been referenced in:
- Trial J et al. Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis. Basic Res Cardiol 112:34 (2017). Read more (PubMed: 28478479) »
- Wu L et al. The CD44 expressed on the earliest intrathymic precursor population functions as a thymus homing molecule but does not bind to hyaluronate. Immunol Lett 38:69-75 (1993). Read more (PubMed: 7507884) »
