Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CD44 antibody [MEM-85] (Biotin)
See all CD44 primary antibodies -
DescriptionMouse monoclonal [MEM-85] to CD44 (Biotin)
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Host speciesMouse
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ConjugationBiotin
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SpecificityThe antibody reacts with both cell surface-expressed and soluble form of CD44 antigen.
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Leucocytes of a patient suffering from LGL Type Leukaemia.
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General notes
The purified antibody is conjugated with Biotin-LC-NHS.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberMEM-85
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IsotypeIgG2b
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/1000. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Sequence similaritiesContains 1 Link domain.
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DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
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Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)](https://www.abcam.com/ps/products/28/ab28105/Images/ab28105-273624-28105.JPG)
Flow Cytometry - Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)Analysis by flow cytometry of ab28105 staining CD44 in human peripheral blood leukocytes.
Protocols
References
ab28105 has not yet been referenced specifically in any publications.
