Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)
Key features and details
- Mouse monoclonal [MEM-85] to CD44 (Biotin)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Overview
-
Product name
Anti-CD44 antibody [MEM-85] (Biotin)
See all CD44 primary antibodies -
Description
Mouse monoclonal [MEM-85] to CD44 (Biotin) -
Host species
Mouse -
Conjugation
Biotin -
Specificity
The antibody reacts with both cell surface-expressed and soluble form of CD44 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Leucocytes of a patient suffering from LGL Type Leukaemia.
-
General notes
The purified antibody is conjugated with Biotin-LC-NHS.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-85 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab28105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/1000. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
-
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
-
![Flow Cytometry - Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)](https://www.abcam.com/ps/products/28/ab28105/Images/ab28105-273624-anti-cd44-antibody-mem-85-biotin-flow-cytometry.jpg)
Flow Cytometry - Anti-CD44 antibody [MEM-85] (Biotin) (ab28105)Analysis by flow cytometry of ab28105 staining CD44 in human peripheral blood leukocytes.
References
ab28105 has not yet been referenced specifically in any publications.
