Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-CD44 antibody [OX-50] (Phycoerythrin)
See all CD44 primary antibodies -
Description
Mouse monoclonal [OX-50] to CD44 (Phycoerythrin) -
Host species
Mouse -
Conjugation
Phycoerythrin. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue/ cell preparation (Rat T cell blasts).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-50 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab23396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25406 Rat
- SwissProt: P26051 Rat
- Unigene: 1120 Rat
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)](https://www.abcam.com/ps/products/23/ab23396/Images/ab23396-219302-anti-cd44-antibody-ox-50-phycoerythrin-flow-cytometry.jpg)
Flow Cytometry - Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)Staining of rat spleen cells with ab23396 (used neat). Top panel: Isotype control. Bottom panel: ab23396.
Protocols
References
This product has been referenced in:
- Orsi PR et al. A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold for mesenchymal stem cells in osteoporotic rats. Stem Cell Res Ther 8:205 (2017). Flow Cyt ; Rat . Read more (PubMed: 28962655) »
- Huang S et al. Systemic Administration of Allogeneic Mesenchymal Stem Cells Does Not Halt Osteoporotic Bone Loss in Ovariectomized Rats. PLoS One 11:e0163131 (2016). Rat . Read more (PubMed: 27711227) »
