Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-CD44 antibody [OX-50] (Phycoerythrin)
See all CD44 primary antibodies -
DescriptionMouse monoclonal [OX-50] to CD44 (Phycoerythrin)
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Host speciesMouse
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ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Rat
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Immunogen
Tissue/ cell preparation (Rat T cell blasts).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberOX-50
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MyelomaNS0/1
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab23396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Sequence similaritiesContains 1 Link domain.
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DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
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Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 25406 Rat
- SwissProt: P26051 Rat
- Unigene: 1120 Rat
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)](https://www.abcam.com/ps/products/23/ab23396/Images/ab23396-219302-ab23396.jpg)
Flow Cytometry - Anti-CD44 antibody [OX-50] (Phycoerythrin) (ab23396)Staining of rat spleen cells with ab23396 (used neat). Top panel: Isotype control. Bottom panel: ab23396.
Protocols
References
This product has been referenced in:
- Orsi PR et al. A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold for mesenchymal stem cells in osteoporotic rats. Stem Cell Res Ther 8:205 (2017). Flow Cyt ; Rat . Read more (PubMed: 28962655) »
- Huang S et al. Systemic Administration of Allogeneic Mesenchymal Stem Cells Does Not Halt Osteoporotic Bone Loss in Ovariectomized Rats. PLoS One 11:e0163131 (2016). Rat . Read more (PubMed: 27711227) »
