Recombinant Anti-CD44 antibody [OX49] (ab238464)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [OX49] to CD44
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Rat
Related conjugates and formulations
Overview
-
Product name
Anti-CD44 antibody [OX49]
See all CD44 primary antibodies -
Description
Mouse monoclonal [OX49] to CD44 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Rat spleen tissue. ICC/IF: C6 cells. Rat splenocytes.Flow Cyt: C6 cells.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
OX49 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.854 µg/ml.
|
|
Flow Cyt |
Use a concentration of 0.474 µg/ml.
|
|
IHC-P |
Use a concentration of 0.085 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 0.854 µg/ml. |
Flow Cyt
Use a concentration of 0.474 µg/ml. |
IHC-P
Use a concentration of 0.085 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 25406 Rat
- SwissProt: P26051 Rat
- Unigene: 1120 Rat
-
Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab238464 at 0.085µg/ml, followed by a ready to use secondary. Membranous staining on rat spleen is observed. The section was incubated with ab238464 for 5 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling CD44 with ab238464 at 0.854µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on C6 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.
The negative controls are as follows:
-ve control 1: ab238464 at 1/500 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat splenocytes labeling CD44 with ab238464 at 0.854µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat splenocytes. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.
The negative controls are as follows:
-ve control 1: ab238464 at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution. -
Flow cytometric analysis of C6 (Rat glial tumor glial cell) cell line labeling CD44 with ab238464 at 0.474µg/ml (red) compared with a Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab238464 has been referenced in 1 publication.
- Zhang Z et al. Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm. BMC Pharmacol Toxicol 23:81 (2022). PubMed: 36273189