Anti-CD44v3 antibody [VFF-327] (ab34229)
Key features and details
- Mouse monoclonal [VFF-327] to CD44v3
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD44v3 antibody [VFF-327] -
Description
Mouse monoclonal [VFF-327] to CD44v3 -
Host species
Mouse -
Specificity
This antibody specifically recognises an epitope encoded by exon V3 on the variant portion of human CD44 -
Tested applications
Suitable for: IHC-Fr, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
VFF-327 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl (at a concentration of 10 µg/ml) for 106 cells in 100µl. |
Notes |
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IHC-Fr
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 10µl for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 10µl (at a concentration of 10 µg/ml) for 106 cells in 100µl. |
Target
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Relevance
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. There are 17 different isoforms. -
Cellular localization
Membrane; single-pass type I membrane protein -
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
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Alternative names
- CD44 antigen antibody
- CD44 molecule antibody
- Heparan sulfate proteoglycan antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab34229 has been referenced in 3 publications.
- Li J et al. Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform. Mol Oncol 13:1993-2009 (2019). PubMed: 31294922
- Alam TN et al. Differential expression of CD44 during human prostate epithelial cell differentiation. J Histochem Cytochem 52:1083-90 (2004). PubMed: 15258184
- Seiter S et al. Mitigation of delayed-type hypersensitivity reactions by a CD44 variant isoform v3-specific antibody: blockade of leukocyte egress. J Invest Dermatol 113:11-21 (1999). PubMed: 10417612