Key features and details
- Mouse monoclonal [VFF-7] to CD44v6
- Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD44v6 antibody [VFF-7]
See all CD44v6 primary antibodies
DescriptionMouse monoclonal [VFF-7] to CD44v6
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IHC-P, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human CD44v6. Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Database link: P16070
EpitopeThis antibody specifically recognises an epitope encoded by exon v6 on the variant portion of human CD44.
- WB: HeLa and A549 cell lysates. IHC-P: Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab30436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/400 - 1/500.|
|WB||Use at an assay dependent concentration.|
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localizationMembrane. Colocalizes with actin in membrane protrusions at wounding edges.
- Information by UniProt
- CD44 antibody
- CD44 antigen antibody
- CD44 isoform(s) antibody
All lanes : Anti-CD44v6 antibody [VFF-7] (ab30436) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CD44 knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : LnCAP cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 80 kDa why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab30436 observed at 80 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab30436 was shown to react with CD44v6 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab30436 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with ab30436 at 1/200 dilution.
ab30436 has been referenced in 5 publications.
- Rezniczek GA et al. Dynamic changes of tumor gene expression during repeated pressurized intraperitoneal aerosol chemotherapy (PIPAC) in women with peritoneal cancer. BMC Cancer 16:654 (2016). PubMed: 27542596
- Li Y et al. Effects of different sutures on fibrosis and wound healing in a rabbit model of corneal wounds. Exp Ther Med 12:2827-2834 (2016). PubMed: 27882081
- Vieira AF et al. P-cadherin is coexpressed with CD44 and CD49f and mediates stem cell properties in basal-like breast cancer. Stem Cells 30:854-64 (2012). PubMed: 22389315
- Liebertz DJ et al. Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1. Head Neck Oncol 2:5 (2010). Flow Cyt . PubMed: 20175927
- Saito H et al. Serum concentration of CD44 variant 6 and its relation to prognosis in patients with gastric carcinoma. Cancer 83:1094-101 (1998). PubMed: 9740073