Anti-CD45 antibody (ab154885)
Key features and details
- Rabbit polyclonal to CD45
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD45 antibody
See all CD45 primary antibodies -
Description
Rabbit polyclonal to CD45 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHuman
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 914-1004 of Human CD45 (UniProt ID: P08575).
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Positive control
- 293T and A431 whole cell lysate. Mouse ESC D3 cells.
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General notes
This antibody provides variable results in WB.
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Entrez Gene: 19264 Mouse
- Omim: 151460 Human
- SwissProt: P08575 Human
- SwissProt: P06800 Mouse
- Unigene: 654514 Human
- Unigene: 391573 Mouse
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (ab154885)Immunofluorescence analysis of paraformaldehyde fixed Mouse ESC D3 cells, labeling CD45 using ab154885 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (ab154885)Immunofluorescence analysis of THP-1 cells labeling CD45 with ab154885 at 1/500 dilution. Hoechst 33342 (blue) staining is used (right panel).
Protocols
References (1)
ab154885 has been referenced in 1 publication.
- Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . PubMed: 25643152
