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  1. Link

    cd45-antibody-bl-178-12c7-ab243869.pdf

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Immunology Adaptive Immunity B Cells CD
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Recombinant

Recombinant Anti-CD45 antibody [BL-178-12C7] (ab243869)

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Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
  • Anti-CD45 antibody [BL-178-12C7] (ab243869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-178-12C7] to CD45
  • Suitable for: WB, IHC-P, ICC, IP, Flow Cyt
  • Reacts with: Human

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Protein
Recombinant human CD45 protein (Fc Chimera Active) (ab214825)

View more associated products

Overview

  • Product name

    Anti-CD45 antibody [BL-178-12C7]
    See all CD45 primary antibodies
  • Description

    Rabbit monoclonal [BL-178-12C7] to CD45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD45 aa 1250-1306 (C terminal). The exact sequence is proprietary. NP_002829.3 and Gene ID 5788.
    Database link: P08575

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituents: 98% Borate buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BL-178-12C7
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development

Associated products

  • Recombinant Protein

    • Recombinant human CD45 protein (Fc Chimera Active) (ab214825)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.
IP
Use at an assay dependent concentration.

Use 20µl/mg lysate.

Flow Cyt
Use 1µl for 106 cells.
Notes
WB
1/1000.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.
IP
Use at an assay dependent concentration.

Use 20µl/mg lysate.

Flow Cyt
Use 1µl for 106 cells.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in disease

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain

    The first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications

    Heavily N- and O-glycosylated.
  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession P08575 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5788 Human
    • Omim: 151460 Human
    • SwissProt: P08575 Human
    • Unigene: 654514 Human
    • Alternative names

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      CD45 was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243869 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243869 at 1/1000 dilution.

      Lane 1: ab243869 IP in Jurkat cell lysate.

      Lane 2: Contol IgG in Jurkat cell lysate.

      Detection: Chemiluminescence with an exposure time of 3 seconds.

    • Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)

      Western blot analysis using ab243869 at 1/1000 dilution.

      Lane 1: Jurkat whole cell lysate (50 µg).

      Lane 2: MOLT-4 whole cell lysate (50 µg).

      Lane3: K562 whole cell lysate (50 µg).

      Lane 4: SR whole cell lysate (50 µg).

      Lane 5: HeLa whole cell lysate (50 µg).

      Lane 6: HEK-293T whole cell lysate (50 µg).

      A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formalin-fixed, paraffin-embedded human small intestine tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
    • Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Flow cytometric analysis of 4% formaldehyde and permeabilized with 90% methanol Jurkat (Human T cell leukemia cell line from peripheral blood) cells labelling CD45 with ab243869 at 1µl/106 cells (Red shaded) compared with a Isotype control (unshaded). DyLight® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.
       
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formalin-fixed, paraffin-embedded human appendix tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
    • Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD45 using ab243869 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
    • Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Anti-CD45 antibody [BL-178-12C7] (ab243869)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab243869? Please let us know so that we can cite the reference in this datasheet.

    ab243869 has not yet been referenced specifically in any publications.

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