Recombinant

Recombinant Anti-CD45 antibody [BL-178-12C7] (ab243869)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-178-12C7] to CD45
  • Suitable for: WB, IHC-P, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD45 antibody [BL-178-12C7]
    See all CD45 primary antibodies

  • Description

    Rabbit monoclonal [BL-178-12C7] to CD45

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD45 aa 1250-1306 (C terminal). The exact sequence is proprietary. NP_002829.3 and Gene ID 5788.
    Database link: P08575

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab243869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • Involvement in disease

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • Domain

    The first PTPase domain interacts with SKAP1.

  • Post-translational
    modifications

    Heavily N- and O-glycosylated.

  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      CD45 was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243869 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243869 at 1/1000 dilution.

      Lane 1: ab243869 IP in Jurkat cell lysate.

      Lane 2: Contol IgG in Jurkat cell lysate.

      Detection: Chemiluminescence with an exposure time of 3 seconds.

    • Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)

      Western blot analysis using ab243869 at 1/1000 dilution.

      Lane 1: Jurkat whole cell lysate (50 µg).

      Lane 2: MOLT-4 whole cell lysate (50 µg).

      Lane3: K562 whole cell lysate (50 µg).

      Lane 4: SR whole cell lysate (50 µg).

      Lane 5: HeLa whole cell lysate (50 µg).

      Lane 6: HEK-293T whole cell lysate (50 µg).

      A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formalin-fixed, paraffin-embedded human small intestine tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
    • Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD45 using ab243869 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)
      Formalin-fixed, paraffin-embedded human appendix tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243869? Please let us know so that we can cite the reference in this datasheet.

    ab243869 has not yet been referenced specifically in any publications.

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