Recombinant Anti-CD45 antibody [EPR27167-58] (ab303670)
![Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR27167-58] (ab303670)](https://www.abcam.com/ps/products/303/ab303670/Images/ab303670-3-anti-cd45-antibody-epr27167-58-immunocytochemistry-human-pbmc.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR27167-58] (ab303670)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27167-58] to CD45
- Suitable for: Flow Cyt, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-CD45 antibody [EPR27167-58]
See all CD45 primary antibodies -
Description
Rabbit monoclonal [EPR27167-58] to CD45 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IPmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Human PBMC cells. Flow Cyt: Human PBMC cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27167-58 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab303670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
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| ICC/IF |
1/500.
|
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
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ICC/IF
1/500. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [EPR27167-58] (ab303670)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD45 with ab303670 at 1/500 dilution (0.938 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic and membranous staining in most human PBMCs. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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![Flow Cytometry - Anti-CD45 antibody [EPR27167-58] (ab303670)](https://www.abcam.com/ps/products/303/ab303670/Images/ab303670-2-anti-cd45-antibody-epr27167-58-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CD45 antibody [EPR27167-58] (ab303670)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CD45 with ab303670 at 1/500 dilution (0.1 µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab303670. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.
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![Anti-CD45 antibody [EPR27167-58] (ab303670)](https://www.abcam.com/ps/products/303/ab303670/Images/ab303670-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD45 antibody [EPR27167-58] (ab303670)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab303670 has not yet been referenced specifically in any publications.