• Product name
    Anti-CD45 antibody [F10-89-4]
    See all CD45 primary antibodies
  • Description
    Mouse monoclonal [F10-89-4] to CD45
  • Host species
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD45. Human T lymphocytes.

  • Positive control
    • IHC-P: Human tonsil (normal) tissue. ICC/IF: Jurkat cells. Flow Cytometry: Jurkat cells.
  • General notes

    Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab30470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.
Flow Cyt 1/10 - 1/50.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr 1/500 - 1/1000.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in disease
    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain
    The first PTPase domain interacts with SKAP1.
  • Post-translational
    Heavily N- and O-glycosylated.
  • Cellular localization
    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all


  • ab30470 staining CD45 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells. The cells were fixed with 4% formaldehyde (10 minutes), then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab30470 at 5 µg/ml (shown in green) and ab206369, Rabbit monoclonal to beta Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). This was followed by an incubation at room temperature for 1 hour with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, at 1 µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 minutes).

  • IHC image of CD45 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab30470, 1 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab30470 (red line). The cells were fixed with 4% paraformaldehyde (10 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30470, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 minutes) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.


This product has been referenced in:
  • Desancé M  et al. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy. Int J Mol Sci 19:N/A (2018). Flow Cyt ; Horse . Read more (PubMed: 29439436) »
  • Liu S  et al. Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients. Oncotarget 7:59877-59891 (2016). Read more (PubMed: 27494883) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


The immunogen for ab30470 CD45 antibody [F10-89-4] is Tissue/ cell preparation of Human T lymphocytes. The epitope has not been mapped.

The antibody is applicable for Flow cytometry. The protocol provided with the testing image on the datasheet indicates no permeabilization was required for the staining. Therefore, it is likely that it detects the extracellular portion of the CD45 receptor.

Read More


After performing an extensive search for leukocyte specific markers, there isn't 1 definitive marker that I can guarantee will only recognize leukocytes and not your cancer cells. With that being said, I have compiled a few options that I think will give you a better chance of having a marker that only recognizes your leukocytes and not your cancer cells based on the list of leukocyte markers given in the link below:

The antibodies that I compiled that I think may give you the best chance of finding a leukocyte-specific marker based on human protein atlas information are listed below:

LAM-1 (SELL, CD62L): http://www.proteinatlas.org/ENSG00000188404/cell
CD62L antibodies:
1. https://www.abcam.com/cd62l-antibody-fmc46-ab11364.html
2. https://www.abcam.com/cd62l-antibody-mel-14-ab119834.html

CD18/CD11: http://www.proteinatlas.org/ENSG00000169896/cell
1. https://www.abcam.com/cd11cd18-antibody-24-ab13219.html
2. https://www.abcam.com/cd18-antibody-clb-lfa-11-54-ab51377.html

I cannot guarantee that these antibodies will not label your cancer cells as well, but I hope the information is helpful to you and that the antibody beyond link listing leukocyte markers and the human protein atlas can help you in your search.

Read More


Below is a link to the human protein atlas database file for CD45, I will forward the alternative specific marker information to you as soon as I compile it.

Read More

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