Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
Key features and details
- Rabbit polyclonal to CD45 - Hematopoietic Stem Cell Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-CD45 antibody - Hematopoietic Stem Cell Marker
See all CD45 primary antibodies -
Description
Rabbit polyclonal to CD45 - Hematopoietic Stem Cell Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig, Monkey
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Detects a band of approximately 155 kDa (predicted molecular weight: 147 kDa).
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| IHC-P | (4) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
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WB
1/500. Detects a band of approximately 155 kDa (predicted molecular weight: 147 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
IHC image of ab10559 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)ab10559 rabbit polyclonal to CD45 (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000).
Expected molecular weight: 147 kDa. WB exposure time: 1min 30sec
Lanes 1 to 10: 20µg of cell lysate per lane
Lanes 6 to 10: ab17550 CD45 blocking peptide used at 1µg/mlLane 1: HeLa Nuclear Extract (ab10559)
Lane 2: HeLa Whole Cell Lysate (ab10559)
Lane 3: A431 Whole Cell Lysate (ab10559)
Lane 4: Jurkat Whole Cell Lysate (ab10559)
Lane 5: HEK293 Whole Cell Lysate (ab10559)
Lane 6: HeLa Nuclear Extract (ab10559+ ab17550)
Lane 7: HeLa Whole Cell Lysate (ab10559+ ab17550)
Lane 8: A431 Whole Cell Lysate (ab10559+ ab17550)
Lane 9: Jurkat Whole Cell Lysate (ab10559+ ab17550)
Lane 10: HEK293 Whole Cell Lysate (ab10559+ ab17550)A strong band, slightly higher was seen in Jurkat cell lysate. The band was blocked using the immunising peptide (ab17550). It is likely that the band is CD45.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab10559 has been referenced in 29 publications.
- Zhu G et al. HOXBLINC long non-coding RNA activation promotes leukemogenesis in NPM1-mutant acute myeloid leukemia. Nat Commun 12:1956 (2021). PubMed: 33782403
- Lu IN et al. Tumor-associated hematopoietic stem and progenitor cells positively linked to glioblastoma progression. Nat Commun 12:3895 (2021). PubMed: 34162860
- Coelho S et al. Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells. Sci Adv 6:eaay8271 (2020). PubMed: 32494604
- Xu H et al. Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction. J Cell Biochem 121:2089-2102 (2020). PubMed: 31736169
- Fontana D et al. Integrated Genomic, Functional, and Prognostic Characterization of Atypical Chronic Myeloid Leukemia. Hemasphere 4:e497 (2020). PubMed: 33196013