Key features and details
- Mouse monoclonal [HI30] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD45 antibody [HI30]
See all CD45 primary antibodies
DescriptionMouse monoclonal [HI30] to CD45
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
- Flow Cyt: Human peripheral blood lymphocytes.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesPurified from tissue culture supernatant.
Light chain typekappa
- Anti-CD45 antibody [HI30] (ab123522)
- Anti-CD45 antibody [HI30] (PE/Cy7®) (ab155340)
- Anti-CD45 antibody [HI30], prediluted (PE/Cy5®) (ab155385)
- Anti-CD45 antibody [HI30] (PE/Cy5.5®) (ab167004)
- Anti-CD45 antibody [HI30] (APC) (ab210270)
- Anti-CD45 antibody [HI30] (FITC) (ab210272)
- Anti-CD45 antibody [HI30] (PE) (ab210273)
- Anti-CD45 antibody [HI30] (redFluor™ 710) (ab242257)
Our Abpromise guarantee covers the use of ab253085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
The antibody has been diluted for use at 5 µL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL.
The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253085 has not yet been referenced specifically in any publications.