Key features and details
- Mouse monoclonal [HI30] to CD45 (PE/Cy7®)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Product nameAnti-CD45 antibody [HI30] (PE/Cy7®)
See all CD45 primary antibodies
DescriptionMouse monoclonal [HI30] to CD45 (PE/Cy7®)
ConjugationPE/Cy7®. Ex: 496nm, Em: 774nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Full length version of the CD45 protein
- Human peripheral blood cells
General notesThis Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Light chain typekappa
- Anti-CD45 antibody [HI30] (ab123522)
- Anti-CD45 antibody [HI30], prediluted (PE/Cy5®) (ab155385)
- Anti-CD45 antibody [HI30] (PE/Cy5.5®) (ab167004)
- Anti-CD45 antibody [HI30] (APC) (ab210270)
- Anti-CD45 antibody [HI30] (FITC) (ab210272)
- Anti-CD45 antibody [HI30] (PE) (ab210273)
- Anti-CD45 antibody [HI30] (redFluor™ 710) (ab242257)
- Anti-CD45 antibody [HI30] (ab253085)
Our Abpromise guarantee covers the use of ab155340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
ab155340 has not yet been referenced specifically in any publications.