redFluor™ 710 Anti-CD45 antibody [HI30] (ab242257)
Key features and details
- redFluor™ 710 Mouse monoclonal [HI30] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: redFluor™ 710
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
redFluor™ 710 Anti-CD45 antibody [HI30]
See all CD45 primary antibodies -
Description
redFluor™ 710 Mouse monoclonal [HI30] to CD45 -
Host species
Mouse -
Conjugation
redFluor™ 710 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
HI30 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-CD45 antibody [HI30] (ab123522)
- PE/Cy7® Anti-CD45 antibody [HI30] (ab155340)
- PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)
- PE/Cy5.5® Anti-CD45 antibody [HI30] (ab167004)
- APC Anti-CD45 antibody [HI30] (ab210270)
- FITC Anti-CD45 antibody [HI30] (ab210272)
- PE Anti-CD45 antibody [HI30] (ab210273)
- violetFluor™ 450 Anti-CD45 antibody [HI30] (ab253085)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
The antibody has been diluted for use at 5 µl per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. The number of cells within a sample should be determined empirically, but typically ranges between 1 x 10e5 to 1 x 10e8 cells. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. The antibody has been diluted for use at 5 µl per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. The number of cells within a sample should be determined empirically, but typically ranges between 1 x 10e5 to 1 x 10e8 cells. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab242257 has not yet been referenced specifically in any publications.