Anti-CD45 antibody [I3/2.3] (ab25386)
- Datasheet
- References (20)
- Protocols
Overview
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Product nameAnti-CD45 antibody [I3/2.3]
See all CD45 primary antibodies -
DescriptionRat monoclonal [I3/2.3] to CD45
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Host speciesRat
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Tested applicationsSuitable for: IP, IHC-Fr, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Epitopeab25386 recognizes a framework epitope present on all CD45 isoforms.
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Positive control
- BALB/c spleen cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
PurityAscites
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Purification notesPurified from ascites.
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ClonalityMonoclonal
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Clone numberI3/2.3
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IsotypeIgG2b
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 17579051 | |
| IHC-P | Use at an assay dependent concentration. PubMed: 19695260 | |
| Flow Cyt | Use at an assay dependent concentration. See Haidl et al. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
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Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
DomainThe first PTPase domain interacts with SKAP1.
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Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
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Database links
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
- Unigene: 391573 Mouse
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [I3/2.3] (ab25386)](https://www.abcam.com/ps/products/25/ab25386/Images/ab25386-291208-ab25386.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [I3/2.3] (ab25386)Wong et al., PLoS, 9(6), e100770, fig6 ,doi: 10.1371/journal.pone.0100770ab25386 staining CD45 in E. coli infected Mouse prostate tissue sections 7 days post-instillation by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was blocked with 10% serum and 1% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in blocking buffer) overnight at 4°C. An Alexa 488 conjugated Donkey polyclonal (1/100) was used as the secondary antibody. CD45 is stained green, Vimentin using ab92547 stained red, Hoechst nuclear stained blue. C is a high magnification of inset box in panel B.
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![Flow Cytometry - Anti-CD45 antibody [I3/2.3] (ab25386)](https://www.abcam.com/ps/products/25/ab25386/Images/ab25386-270961-ab25386FC.jpg)
Flow Cytometry - Anti-CD45 antibody [I3/2.3] (ab25386)Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25386 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-PE.
References
This product has been referenced in:
- Graham-Gurysh E et al. Sustained Delivery of Doxorubicin via Acetalated Dextran Scaffold Prevents Glioblastoma Recurrence after Surgical Resection. Mol Pharm 15:1309-1318 (2018). Read more (PubMed: 29342360) »
- Jurisic A et al. Pravastatin and-L-arginine combination improves umbilical artery blood flow and neonatal outcomes in dichorionic twin pregnancies through an nitric oxide-dependent vasorelaxant effect. Vascul Pharmacol N/A:N/A (2018). Read more (PubMed: 29879462) »
