Anti-CD45 antibody [I3/2.3] (ab25386)
![Flow Cytometry - Anti-CD45 antibody [I3/2.3] (ab25386)](https://www.abcam.com/ps/products/25/ab25386/Images/ab25386-270961-anti-cd45-antibody-i3-23-flow-cytometry.jpg "Flow Cytometry - Anti-CD45 antibody [I3/2.3] (ab25386)")
Key features and details
- Rat monoclonal [I3/2.3] to CD45
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-CD45 antibody [I3/2.3]
See all CD45 primary antibodies -
Description
Rat monoclonal [I3/2.3] to CD45 -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD45. T1M1 (Thy-1─c) cells
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Epitope
ab25386 recognizes a framework epitope present on all CD45 isoforms. -
Positive control
- BALB/c spleen cells.
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General notes
This product was changed from ascites to tissue culture supernatant on (28 March 2019). Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
I3/2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
See Haidl et al. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. See Haidl et al. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
- Unigene: 391573 Mouse
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Flow Cytometry - Anti-CD45 antibody [I3/2.3] (ab25386)
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25386 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-PE.
This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab25386 has been referenced in 37 publications.
- Mori A et al. Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models. Cells 10:N/A (2021). PubMed: 33672150
- Pluchino N et al. CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking. J Cell Mol Med 24:2464-2474 (2020). PubMed: 31904910
- Martinez-Corral I et al. Blockade of VEGF-C signaling inhibits lymphatic malformations driven by oncogenic PIK3CA mutation. Nat Commun 11:2869 (2020). PubMed: 32513927
- Archacka K et al. Beneficial Effect of IL-4 and SDF-1 on Myogenic Potential of Mouse and Human Adipose Tissue-Derived Stromal Cells. Cells 9:N/A (2020). PubMed: 32560483
- Pérez-Hernández M et al. Transcriptomic Coupling of PKP2 With Inflammatory and Immune Pathways Endogenous to Adult Cardiac Myocytes. Front Physiol 11:623190 (2020). PubMed: 33536940