Product nameAnti-CD45 antibody [MEM-28] (Alexa Fluor® 647)
See all CD45 primary antibodies
DescriptionMouse monoclonal [MEM-28] to CD45 (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Does not react with: Horse
Tissue, cells or virus corresponding to CD45. (Human thymocytes and T lymphocytes).
- Flow Cyt: Human peripheral blood cells.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Concentration information loading...
Purification notesPurified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Anti-CD45 antibody [MEM-28], prediluted (FITC) (ab134199)
- Anti-CD45 antibody [MEM-28], prediluted (Phycoerythrin) (ab134202)
- Anti-CD45 antibody [MEM-28] (PerCP/Cy5.5®) (ab157309)
- Anti-CD45 antibody [MEM-28] (Allophycocyanin/Cy7 ®) (ab197130)
- Anti-CD45 antibody [MEM-28] (PE-DyLight™ 594) (ab223183)
- Anti-CD45 antibody [MEM-28] (PE/Cy7 ®) (ab239317)
- Anti-CD45 antibody [MEM-28] (Allophycocyanin) (ab28106)
- Anti-CD45 antibody [MEM-28] (Biotin) (ab28107)
- Anti-CD45 antibody [MEM-28] (PerCP) (ab65952)
- Anti-CD45 antibody [MEM-28] (ab8216)
Our Abpromise guarantee covers the use of ab239276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µl for 106 cells.
(or 100 µl of whole blood).
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
ab239276 has not yet been referenced specifically in any publications.