Product nameAnti-CD45 antibody [MEM-28] (PerCP)
See all CD45 primary antibodies
DescriptionMouse monoclonal [MEM-28] to CD45 (PerCP)
ConjugationPerCP. Ex: 482nm, Em: 675nm
Specificityab65952 reacts with all alternative forms of human CD45 antigen.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue/ cell preparation;Human thymocytes and T lymphocytes.
- Flow Cyt: Human blood cells.
The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notespurity >95% by PAGE-PAGE
- Anti-CD45 antibody [MEM-28], prediluted (FITC) (ab134199)
- Anti-CD45 antibody [MEM-28], prediluted (Phycoerythrin) (ab134202)
- Anti-CD45 antibody [MEM-28] (PerCP/Cy5.5®) (ab157309)
- Anti-CD45 antibody [MEM-28] (APC/Cy7®) (ab197130)
- Anti-CD45 antibody [MEM-28] (PE-DyLight™ 594) (ab223183)
- Anti-CD45 antibody [MEM-28] (Alexa Fluor® 647) (ab239276)
- Anti-CD45 antibody [MEM-28] (PE/Cy7 ®) (ab239317)
- Anti-CD45 antibody [MEM-28] (Allophycocyanin.) (ab28106)
- Anti-CD45 antibody [MEM-28] (Biotin) (ab28107)
- Anti-CD45 antibody [MEM-28] (ab8216)
Our Abpromise guarantee covers the use of ab65952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use 10µl of neat solution for 106 cells or 100µl of whole blood
ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
ab65952 has been referenced in 4 publications.
- Gholizadeh-Ghaleh Aziz S et al. Human amniotic fluid stem cells (hAFSCs) expressing p21 and cyclin D1 genes retain excellent viability after freezing with (dimethyl sulfoxide) DMSO. Bosn J Basic Med Sci 19:43-51 (2019). PubMed: 29688163
- Shao B et al. Regulatory effects of miRNA-181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency-induced osteoporosis. Mol Med Rep 18:920-930 (2018). PubMed: 29845202
- Gholizadeh-Ghaleh Aziz S et al. Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells. PLoS One 11:e0158281 (2016). Flow Cyt ; Human . PubMed: 27434028
- Sadallah S et al. Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells. J Immunol 186:6543-52 (2011). Flow Cyt ; Human . PubMed: 21525379