• Product name
    Anti-CD45 antibody [MRC OX-1]
    See all CD45 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-1] to CD45
  • Host species
  • Specificity
    ab33923 recognises CD45, also known as the leucocyte common antigen (LCA).
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.


Our Abpromise guarantee covers the use of ab33923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100.

Use 10ul of the suggested working dilution to label 106 cells in 100ul.




ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.


  • Function
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in disease
    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain
    The first PTPase domain interacts with SKAP1.
  • Post-translational
    Heavily N- and O-glycosylated.
  • Cellular localization
    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all


This product has been referenced in:
  • Mangas A  et al. Gemst: a taylor-made combination that reverts neuroanatomical changes in stroke. Eur J Histochem 61:2790 (2017). Read more (PubMed: 28735520) »
  • Ortiz-Masiá D  et al. iNOS-derived nitric oxide mediates the increase in TFF2 expression associated with gastric damage: role of HIF-1. FASEB J 24:136-45 (2010). IHC-P ; Rat . Read more (PubMed: 19741170) »

See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain)
Antigen retrieval step
Yes - Triton 0.5%
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 73°C

Abcam user community

Verified customer

Submitted Sep 26 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (head and neck cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
head and neck cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 19 2016

Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456.

Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, ...

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Vielen Dank für Ihre Geduld.

Es ist tatsächlich so, dass wir diesen Antikörper unter verschiedenen Bedingungen nicht in der IHC-P zum Laufen gebracht habe. Zwar haben wir die Publikation in welcher er in IHC-P verwendet wird, aber wir haben ...

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Mir ist aufgefallen, dass internen Daten bezüglich der Reaktivität dieses Antikörpers in der IHC-P widersprüchlich sind: Angeblich funktionierte dieser Antikörper in unseren Händen nicht in der IHC-P. Diese Applikation wurde später automatisch durch un...

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Thank you for your reply.

Your credit note ID is 00000.

This can be used for the bill you received for this antibody. Please let our finance department (mailto:creditcontrol@abcam.com) know how you would like to use it.


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Thank you for contacting us and sorry for the delay in getting back to you.

The anti-CD45 antibody(ab33923) has been used with rat samples with paraffin embedded tissue sections. Please refer to the reference below:


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Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.
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