Anti-CD45 antibody [MRC OX-1] (ab33923)
Key features and details
- Mouse monoclonal [MRC OX-1] to CD45
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD45 antibody [MRC OX-1]
See all CD45 primary antibodies -
Description
Mouse monoclonal [MRC OX-1] to CD45 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD45.
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Positive control
- IHC-Fr: Mouse and rat spleen tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-1 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 1 µg/ml. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 19264 Mouse
- Entrez Gene: 24699 Rat
- SwissProt: P06800 Mouse
- SwissProt: P04157 Rat
- Unigene: 391573 Mouse
- Unigene: 90166 Rat
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (ab33923)](https://www.abcam.com/ps/products/33/ab33923/Images/ab33923-2-ab33923IHCFr2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (ab33923)IHC image of CD45 staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
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![Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (ab33923)](https://www.abcam.com/ps/products/33/ab33923/Images/ab33923-1-ab33923IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (ab33923)IHC image of CD45 staining in a section of frozen normal mouse spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using the rodent block from Mouse on Mouse detection kit ab127055 for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
References (5)
ab33923 has been referenced in 5 publications.
- Mangas A et al. Gemst: a taylor-made combination that reverts neuroanatomical changes in stroke. Eur J Histochem 61:2790 (2017). PubMed: 28735520
- Vargas-Caraveo A et al. Chronic Psychological Distress as an Inducer of Microglial Activation and Leukocyte Recruitment into the Area Postrema. Neuroimmunomodulation 22:311-21 (2015). PubMed: 25765708
- Ortiz-Masiá D et al. iNOS-derived nitric oxide mediates the increase in TFF2 expression associated with gastric damage: role of HIF-1. FASEB J 24:136-45 (2010). IHC-P ; Rat . PubMed: 19741170
- Vansthertem D et al. Label-retaining cells and tubular regeneration in postischaemic kidney. Nephrol Dial Transplant : (2008). PubMed: 18678561
- Alvarez A et al. Gastric antisecretory drugs induce leukocyte-endothelial cell interactions through gastrin release and activation of CCK-2 receptors. J Pharmacol Exp Ther 323:406-13 (2007). PubMed: 17652263
