Overview

  • Product name

    Anti-CD45 antibody [MRC OX-1]
    See all CD45 primary antibodies
  • Description

    Mouse monoclonal [MRC OX-1] to CD45
  • Host species

    Mouse
  • Specificity

    ab33923 recognises CD45, also known as the leucocyte common antigen (LCA).
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD45.

  • Positive control

    • IHC-Fr: Mouse and rat spleen tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Applications

Our Abpromise guarantee covers the use of ab33923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in disease

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain

    The first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications

    Heavily N- and O-glycosylated.
  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Images

  • IHC image of CD45 staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.

    Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

  • IHC image of CD45 staining in a section of frozen normal mouse spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.

    Non-specific protein-protein interactions were blocked using the rodent block from Mouse on Mouse detection kit ab127055 for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

References

This product has been referenced in:

  • Mangas A  et al. Gemst: a taylor-made combination that reverts neuroanatomical changes in stroke. Eur J Histochem 61:2790 (2017). Read more (PubMed: 28735520) »
  • Vargas-Caraveo A  et al. Chronic Psychological Distress as an Inducer of Microglial Activation and Leukocyte Recruitment into the Area Postrema. Neuroimmunomodulation 22:311-21 (2015). Read more (PubMed: 25765708) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton 0.5%
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 73°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (head and neck cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization
No
Specification
head and neck cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2016

Answer

Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456.

Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutschrift für Sie auszustellen. Sie können diese Gutschrift auf folgende Weise verwenden:

(1) Gegen die Rechnung dieses Antikörpers verwenden falls diese noch nicht bezahlt wurde
(2) Aufbewahren und gegen eine zukünftige Rechnung verwenden
(3) Wir stellen Ihnen gerne eine komplette Rückerstattung aus, wenn keine offenen Rechnungen mehr ausstehen

Um eine Rückerstattung des Kaufpreises zu erhalten oder um zu bestätigen wie Sie diese Gutschrift benutzen möchten, möchte ich Sie bitten, sich mit unserer Finanzabteilung in Verbindung zu setzen.
Sie können unsere Finanzabteilung per Email unter der Adresse creditcontrol@abcam.com oder telefonisch unter der Nummer 030 896 779 154 erreichen.

Bitte geben Sie die Referenznummer Ihrer Gutschrift immer mit an.

Die Referenznummer ist für Ihre Unterlagen bestimmt. Wir schicken Ihnen die eigentliche Gutschrift per Post oder Email zu. Sie enthält auch die Gutschriftnummer, die mit dem Buchstaben CGB beginnt.

Ich hoffe dass Sie trotz dieser schlechten Erfahrung weiterhin Kunde bei uns bleiben. Bitte zögern Sie nicht sich wieder an uns zu wenden, falls Sie weitere Fragen haben.

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Answer

Vielen Dank für Ihre Geduld.

Es ist tatsächlich so, dass wir diesen Antikörper unter verschiedenen Bedingungen nicht in der IHC-P zum Laufen gebracht habe. Zwar haben wir die Publikation in welcher er in IHC-P verwendet wird, aber wir haben die Applikation vom Datenblatt entfernt.


Da Sie wie gesagt von unserer Garantie abgedeckt sind, können wir Ihnen entweder einen kostenlosen Ersatz, eine Gutschrift oder eine Rückerstattung zukommen lassen.


Ich hoffe, dies hilft Ihnen weiter. Bitte lassen Sie mich wissen, was für Sie in Frage kommt.

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Answer

Mir ist aufgefallen, dass internen Daten bezüglich der Reaktivität dieses Antikörpers in der IHC-P widersprüchlich sind: Angeblich funktionierte dieser Antikörper in unseren Händen nicht in der IHC-P. Diese Applikation wurde später automatisch durch unsere Suchmaschine anhand einer Publikation hinzugefügt. Soweit ich mehr Informationen aus unserem Labor erhalten habe, werde ich mich wieder bei ihnen melden. Vielleicht waren unsere Testbedingungen einfach nicht ideal?

Falls diese Applikation niemals auf das Datenblatt hätte kommen dürfen, sind Sie selbstverständlich immer noch von unserer Garantie abgedeckt, da es sich dann natürlich um unseren Fehler handelt.

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Answer

Thank you for your reply.

Your credit note ID is 00000.

This can be used for the bill you received for this antibody. Please let our finance department (mailto:creditcontrol@abcam.com) know how you would like to use it.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Answer

Thank you for contacting us and sorry for the delay in getting back to you.

The anti-CD45 antibody(ab33923) has been used with rat samples with paraffin embedded tissue sections. Please refer to the reference below:

Ortiz-Masiá D et al. iNOS-derived nitric oxide mediates the increase in TFF2 expression associated with gastric damage: role of HIF-1. FASEB J 24:136-45 (2010). PubMed: 19741170

The antibody would be covered by the Abpromise guarantee if you were to use this antibody in this application and species. For more information on the Abpromise guarantee please refer tothe link below:

https://www.abcam.com/abpromise

If the CD45 protein is expressed in the macrophages of the tissue then these should be detected by this antibody. I have found reference to this protein being expressed in marcophages:

http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0007475

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.

1-ab33923 - IHC-P: 1/50
2-ab6668 - ICC/IF: 1/1000, WB: 1/2000, ELISA: 1/50000
3-ab6822 - WB: 1/2000, ELISA: 1/200000, ICC/IF: 1/1000, IHC-P:1/100
4-ab7481 -10%
5-ab97049 - IHC-P: 1/500, ICC/IF: 1/200, ELISA: 1/20000, WB: 1/10000.

Ifthere is anythingelse I can help you with, or ifyou would like a starting concentration for an application that I did not list about, then please let me know.

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