- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-CD45 antibody [MRC OX-1]
See all CD45 primary antibodies -
DescriptionMouse monoclonal [MRC OX-1] to CD45
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Host speciesMouse
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Specificityab33923 recognises CD45, also known as the leucocyte common antigen (LCA).
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Frmore details
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Species reactivityReacts with: Rat
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Immunogen
Rat thymocyte membrane glycoproteins.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberMRC OX-1
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MyelomaNS1
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab33923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
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Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
DomainThe first PTPase domain interacts with SKAP1.
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Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
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Database links
- Entrez Gene: 24699 Rat
- SwissProt: P04157 Rat
- Unigene: 90166 Rat
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Protocols
References
This product has been referenced in:
- Mangas A et al. Gemst: a taylor-made combination that reverts neuroanatomical changes in stroke. Eur J Histochem 61:2790 (2017). Read more (PubMed: 28735520) »
- Ortiz-Masiá D et al. iNOS-derived nitric oxide mediates the increase in TFF2 expression associated with gastric damage: role of HIF-1. FASEB J 24:136-45 (2010). IHC-P ; Rat . Read more (PubMed: 19741170) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"