Anti-CD45 antibody [OX30] (ab6329)

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Overview

  • Product name

    Anti-CD45 antibody [OX30]
    See all CD45 primary antibodies

  • Description

    Mouse monoclonal [OX30] to CD45

  • Host species

    Mouse

  • Specificity

    Reacts with all molecular forms of rat CD45, the Leucocyte Common Antigen (LCA).

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Tissue, cells or virus corresponding to Rat CD45. Rat lymph node cells and glycoproteins.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Applications

Our Abpromise guarantee covers the use of ab6329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.2 µg/ml.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • Involvement in disease

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • Domain

    The first PTPase domain interacts with SKAP1.

  • Post-translational
    modifications

    Heavily N- and O-glycosylated.

  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)
      Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)Lab

      Lewis rat splenocytes stained with ab6329 (right) or mouse IgG2aκ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6329) or mouse IgG2aκ Isotype (ab18450) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

      The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

      Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

       

    References

    This product has been referenced in:

    • Woollett GR  et al. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur J Immunol 15:168-73 (1985). Read more (PubMed: 2578966) »
    See 1 Publication for this product

    Publishing research using ab6329? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    Our customer wants to know the positive control of ab6329. She will perform IHC. Could you recommend the positive control?

    Read More
    Answer

    Thank you for your enquiry. Whilst we do not have a recommended positive control the following publication employed leukocytes as a control for the detection of CD45 transcripts in rat cells. De Dios I, Ramudo L, Alonso JR, Recio JS, Garcia-Montero AC, Manso MA. CD45 expression on rat acinar cells: involvement in pro-inflammatory cytokine production. FEBS Lett. 2005 Nov 21;579(28):6355-60. Epub 2005 Oct 24. PMID: 16263122 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Please comment on the suitability of these five antibodies for use in IHC-P on rat tissue.

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    Answer

    You recently spoke with my colleague Maud Osborne regarding the use of a number of anti CD antibodies in IHC on paraffin embedded rat sections. As you have seen, none of the antibodies had been tested in the exact conditions you wish to try although my comments about their use are below. ab26239 (CD44) - has been tested in IHC-P on mouse & human although not yet tested in rat. As it has been shown to work in mouse I think it's a good bet that it will work in rat although there's no guarantee. ab6329 (CD45) - has been tested in IHC on Frozen sections but not on paraffin sections and shown to cross react with rat. ab22479 (CD45) - raised in rat and shown to cross react with rat - take care when using this antibody in IHC to block any endogenous rat IgGs that may bind to your anti rat secondary ab23844 (CD44) - works in IHC-P and cross reacts with cow, dog, goat, horse, pig, sheep so again although there's no guarantee, I think it's reasonable to think that this will cross react with rat too! ab19673 (CD105) - cross reacts with human and works in IHC-P I hope this is a helpful summary.

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    Question

    Has this antibody been tested in IHC-paraffin or IHC-frozen, or both?

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    Answer

    Thank you for your patience. This product has been tested in IHC-frozen, used at dilutions between 1:25 - 1:200. I have updated the datasheet to reflect this information for future use. To the best of my knowledge, it has not been tested in IHC-paraffin. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Is this a mouse or rat monoclonal? In the raised in section is says rat, but the description is mouse monoclonal. If rat, has it been tested in formalin fixed paraffin embedded sections?

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    Answer

    Unfortunately, MAS 703 has not been tested on formalin fixed tissue. The odds are that it will work, but we can't say for certain until tested.

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    Question

    Do you have any info regarding cross reactivity with mice tissue? Does it work on formalin fixed, paraffin embedded tissue? How long do you incubate in this primary? Does this require antigen retrieval? If so, what is the method/buffer/enzyme you use? Thank-you

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    Answer

    This antibody is raised in mouse and so is difficult to use on mouse tissue without getting problems with background staining. We have no records of this antibody working on paraffin sections.

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