Key features and details
- Rat monoclonal [YTH24.5] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-CD45 antibody [YTH24.5]
See all CD45 primary antibodies
DescriptionRat monoclonal [YTH24.5] to CD45
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD45.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesImmunoglobulin fraction prepared by ammonium sulphate precipitation.
Our Abpromise guarantee covers the use of ab30446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD 45 antibody
- CD45 antibody
Overlay histogram showing peripheral blood lymphocytes stained with ab30446 (red line). The cells were incubated with the antibody (ab30446, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
ab30446 has been referenced in 6 publications.
- Yao Y et al. Exosomes derived from mesenchymal stem cells reverse EMT via TGF-ß1/Smad pathway and promote repair of damaged endometrium. Stem Cell Res Ther 10:225 (2019). PubMed: 31358049
- Yuan K et al. The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation. Stem Cells Int 2019:5310202 (2019). PubMed: 31885614
- Ishibashi R et al. Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement. Oncol Lett 18:6397-6404 (2019). PubMed: 31807163
- Boyd AL et al. Niche displacement of human leukemic stem cells uniquely allows their competitive replacement with healthy HSPCs. J Exp Med 211:1925-35 (2014). PubMed: 25180064
- Peterson VM et al. Ascites analysis by a microfluidic chip allows tumor-cell profiling. Proc Natl Acad Sci U S A 110:E4978-86 (2013). Human . PubMed: 24297935
- Vangeepuram N et al. Processing of antibodies bound to B-cell lymphomas and lymphoblastoid cell lines. Cancer 80:2425-30 (1997). PubMed: 9406693