Key features and details
- Mouse monoclonal [A20] to CD45.1
- Suitable for: Flow Cyt, ICC/IF, IP
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-CD45.1 antibody [A20]
See all CD45.1 primary antibodies
DescriptionMouse monoclonal [A20] to CD45.1
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IPmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
ab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer100mM borate buffered saline, pH 8.0.
Concentration information loading...
Primary antibody notesab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.
Light chain typekappa
- Anti-CD45.1 antibody [A20] (PerCP/Cy5.5®) (ab210237)
- Anti-CD45.1 antibody [A20] (violetFluor™ 450) (ab241939)
- Anti-CD45.1 antibody [A20] (FITC) (ab24917)
- Anti-CD45.1 antibody [A20] (Cy5 ®) (ab24982)
- Anti-CD45.1 antibody [A20] (Biotin) (ab25011)
- Anti-CD45.1 antibody [A20] (APC) (ab25348)
- Anti-CD45.1 antibody [A20] (PE/Cy5®) (ab25349)
- Anti-CD45.1 antibody [A20] (APC/Cy5.5®) (ab81989)
Our Abpromise guarantee covers the use of ab25078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
RelevanceCD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
Cellular localizationType I membrane protein.
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
ab25078 has been referenced in 4 publications.
- Wang L et al. Efficient tumor regression by adoptively transferred CEA-specific CAR-T cells associated with symptoms of mild cytokine release syndrome. Oncoimmunology 5:e1211218 (2016). PubMed: 27757303
- Soehnlein O et al. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Mol Med 5:471-81 (2013). IHC-Fr ; Mouse . PubMed: 23417922
- Antoine-Moussiaux N et al. A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death. PLoS One 4:e5728 (2009). Mouse . PubMed: 19478957
- Sitati E et al. CD40-CD40 ligand interactions promote trafficking of CD8+ T cells into the brain and protection against West Nile virus encephalitis. J Virol 81:9801-11 (2007). IHC-FoFr ; Mouse . PubMed: 17626103