Overview

  • Product name
    Anti-CD45.1 antibody [A20]
    See all CD45.1 primary antibodies
  • Description
    Mouse monoclonal [A20] to CD45.1
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-FoFr, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    ab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17626103
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance
    CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • Cellular localization
    Type I membrane protein.
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • LY5 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Images

  • Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/106 cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

References

This product has been referenced in:
  • Soehnlein O  et al. Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes. EMBO Mol Med 5:471-81 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23417922) »
  • Antoine-Moussiaux N  et al. A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death. PLoS One 4:e5728 (2009). Mouse . Read more (PubMed: 19478957) »
See all 3 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your call last week. My colleague who you spoke to on Friday is out of the office today, but I will be happy to assist you with this issue. I've created the discount code below. Please let me know if there is anything else that we can do for you.

DISCOUNT CODE: ***
Expiration date: November 10, 2012

I am very pleased to hear you would like to accept our offer and test ab25078 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab25078 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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