Overview

  • Product name

    Anti-CD45.1 antibody [A20] (violetFluor™ 450)
    See all CD45.1 primary antibodies
  • Description

    Mouse monoclonal [A20] to CD45.1 (violetFluor™ 450)
  • Host species

    Mouse
  • Conjugation

    violetFluor™ 450
  • Specificity

    ab241939 has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Flow Cyt: SJL splenocytes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
  • Clonality

    Monoclonal
  • Clone number

    A20
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use µl for 10 cells.

Use 0.5 µg/test.

Target

  • Relevance

    CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • Cellular localization

    Type I membrane protein.
  • Database links

  • Alternative names

    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • LY5 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Images

  • Flow cytometric analysis of SJL splenocytes labeling CD45.1 with 0.5 µg ab241939 (solid line) or 0.5 µg violetFluor™ 450 Mouse IgG2a isotype control (dashed line).

References

ab241939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241939.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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