Key features and details
- Rat monoclonal [RA3-6B2] to CD45R
- Suitable for: IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-CD45R antibody [RA3-6B2]
See all CD45R primary antibodies
DescriptionRat monoclonal [RA3-6B2] to CD45R
Tested applicationsSuitable for: IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Abelson murine leukemia virus-induced pre-B tumor cells
- C57BL/6 mouse splenocytes
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab64100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.25µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
ab64100 at 0.25µg per 106 cells in 100µl staining C57BL/6 mouse splenocytes followed by anti-rat IgG FITC.
ab64100 staining CD45R in mouse lympn node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 25 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in 1% BSA/TPBS) for 16 hours at 25°C. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
ab64100 staining CD45R in mouse bone marrow cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/500) was used as the secondary antibody.
ab64100 staining CD45R in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/200 dilution. Nuclei were counterstained with DAPI.
ab64100 has been referenced in 41 publications.
- Nicolas L et al. Cutting Edge: ATM Influences Germinal Center Integrity. J Immunol 202:3137-3142 (2019). PubMed: 31028119
- Bolsega S et al. Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice. Front Immunol 10:1708 (2019). PubMed: 31396223
- Klinngam W et al. Inhibition of Cathepsin S Reduces Lacrimal Gland Inflammation and Increases Tear Flow in a Mouse Model of Sjögren's Syndrome. Sci Rep 9:9559 (2019). PubMed: 31267034
- Wang Z et al. Upregulation of proBDNF in the Mesenteric Lymph Nodes in Septic Mice. Neurotox Res 36:540-550 (2019). PubMed: 31278527
- Dillon MT et al. ATR Inhibition Potentiates the Radiation-induced Inflammatory Tumor Microenvironment. Clin Cancer Res 25:3392-3403 (2019). PubMed: 30770349